Objective To study the effect of maternal high sucrose exposure on Ang II -induced vasoconstriction of mesenteric arteries in adult offspring rats. Methods The pregnant Sprague-Dawley (SD) rats were randomly divided into two groups; High sucrose group(HS) rats were given 20% (W/V) sucrose solution and standard rat food, control group(C) rats were provided with fresh tap water and stan dard rat food, The mesenteric arteries from the adult offspring in both groups were used for experiments. Vascular tone induced by Ang Ⅱ in absence or presence of charybdotoxin( CTX) and Ca2+ in single mesenteric artery were measured in the same time. Protein abundance of large-conductance calcium activated-potassium channel ( BK) a-subunits in mesenteric arteries of adult offspring rats were measured with Western blot. Results In HS group, Ang Ⅱ -induced contractions of the mesenteric artery were significantly increased compared with that in control group (P <0.01); Inhibition of BK with CTX significantly decreased the Ang Ⅱ -induced maximal response ( P < 0.05 ). HS group displayed higher Ca2 + (P < 0. 01) and clearly decreased BK a-subunits protein expression levels, as compared with control group(P< 0. 01). Conclusion Prenatal high sucrose exposure enhance Ang Ⅱ -induced vessel tone in adult offspring attributing to increased Ca2+ and functional decrease of BK channels in mesenteric arteris. The "imprinting" mechanism in detail needs to be studied further.
prenatal high sucroseadult offspringmesenteric arteryangiotensin ⅡBK channel
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NSTL
维普
万方数据
