Objective To determine the effect of high-salt diet during pregnancy on angiotensin-re-lated cardiac cell cycles. Methods Pregnant Sprague-Dawley rats were randomized to either normal-salt diet or high-salt diet during gestational day 3 ~ 21. Blood samples from maternal and fetal rats were prepared for measuring sodium,osmolality,and the hormones. Fetal heart weight was measured. Cardiac cells were cultured for analyzing cell cycle. Results There was no significantly difference of blood sodium concentrations and osmolality in both maternal and fetal rats between the control and high-salt group (all P >0.05). High-salt diet caused plasma Ang Ⅱ decreased in both the mothers and fetuses, and Ang Ⅱ in the fetal myocardium was significantly increased ( all P > 0.05). Gl phase percentage was significantly decreased, whereas S phase percentage was increased in the heart cells from the high salt group compared with those from the control group (all P > 0.05 ). Ang Ⅱ decreased Gl phase percentage and increased S phase percentage in the cardiac cells. Losartan,AT,R antagonist, reversed the changes of Gl and S phases induced by Ang Ⅱ. PD123319, AT2R antagonist, did not block the changes induced by Ang Ⅱ in the cardiac cells. Conclusion High-salt diet during pregnancy may activate the local Ang Ⅱ in fetal rats heart. The effect of cell cycles was mediated by AT,R.
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