冬凌草甲素对人乳腺癌细胞侵袭的抑制作用及机制
Inhibition of the invasion of human breast cancer cells by oridonin and the underlying mechanims
彭延延 1施秋萍 1杨大朋 1王海啸 1马长艳1
作者信息
- 1. 南京医科大学发育遗传学系,江苏南京210029
- 折叠
摘要
目的 检测冬凌草甲素对人乳腺癌细胞MDA-MB 231侵袭的影响并探讨其作用的分子机制.方法 用Transwell实验检测冬凌草甲素对人乳腺癌细胞MDA-MB 231侵袭的影响；明胶酶谱实验检测冬凌草甲素对基质金属蛋白酶-2 (MMP-2)和基质金属蛋白酶-9(MMP-9)酶活性的影响；荧光素酶报告基因实验检测冬凌草甲素对MMP-2和MMP-9启动子活性的影响.结果 冬凌草甲素明显抑制MDA-MB 231细胞的侵袭,且明显下调MMP-2和MMP-9的启动子活性和酶活性(P＜0.05或＜0.01).结论 冬凌草甲素能显著抑制乳腺癌细胞的侵袭,且这种抑制作用可能通过下调MMP-2和MMP-9的启动子活性和酶活性而实现.
Abstract
Objective To investigate the effects of oridonin on the invasion of human breast cancer cells MDA-MB 231 and the underlying mechanisms. Methods Transwell assay was performed to examine the effects of oridonin on the invasion of human breast cancer cells MDA-MB 231. Gelatin zymography assay was performed to detect the effects of oridonin on the enzyme activities of MMP-2 and MMP-9. Lu-ciferase reporter assay was carried out to test the effects of oridonin on the promoter activities of MMP-2 and MMP-9. Results Oridonin significantly inhibited the invasion of MDA-MB 231 cells. And oridonin treatment led to the decrease of the promoter activities and enzyme activities of MMP-2 and MMP-9 (P < 0. 05 or P <0.01). Conclusion Oridonin is able to effectively inhibit the invasion of breast cancer cells and this inhibition may be reached through decreasing the promoter activities and enzyme activities of MMP-2 and MMP-9.
关键词
冬凌草甲素/乳腺癌/侵袭/明胶酶谱/荧光素酶报告基因分析Key words
oridonin/breast cancer/invasion/gelatin zymography/luciferase reporter assay引用本文复制引用
基金项目
国家自然科学基金(81071525)
2011年江苏高校大学生实践创新训练计划项目()
2012年江苏省六大人才高峰资助项目(DG216D5048)
出版年
2012
NETL
NSTL
维普
万方数据