腺病毒载体介导IL-24基因对肺癌细胞的化疗增敏效应
Recombinant adenovirus expressing IL-24 enhances the sensitivity of lung cancer cell to chemotherapeutic drugs
李畅 1赵军 1李印 2梁光辉 2盛伟华 3杨吉成3
作者信息
- 1. 苏州大学附属第一医院心胸外科,江苏苏州215006
- 2. 河南省肿瘤医院胸外科,河南郑州450052
- 3. 苏州大学医学部基础医学与生物科学学院细胞与分子生物学教研室,江苏苏州215123
- 折叠
摘要
目的 研究携带人IL-2基因腺病毒载体(Ad-IL-24)联用顺铂(DDP)对人非小细胞肺癌NCI-H460细胞生长和凋亡的影响.方法 取对数生长期的NCI-H460细胞,MTT比色法检测Ad-IL-24联合DDP对细胞生长的影响,流式细胞仪分析细胞周期及检测细胞凋亡率.结果 Ad-IL-24联合DDP对NCI-H460细胞的生长抑制作用和诱导凋亡效应均明显优于单用Ad-IL-24和单用化疗药物DDP,且G2/M期细胞比例显著增加(P＜0.05或＜0.01).结论 Ad-IL-24能提高NCI-H460肺癌细胞对化疗药物DDP的敏感性.
Abstract
Objective To investigate the effects of recombinant adenovirus expressing IL-24 (Ad-IL-24) combined with cisplatin(DDP) on growth and apoptosis of human non-small cell lung cancer NCI-H460 cells. Methods NCI-H460 cells in logarithmic phase were prepared. Effects of Ad-IL-24 combined with DDP on cell growth of NCI-H460 cells were detected by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry. Results Compared with any single group, the inhibition of cell growth and cell apoptosis in combined group are more effective. Furthermore, Ad-IL-24 combined with DDP significantly increased the ratio of cells in G2/M phase(p<0.05 or <0.01). Conclusion Recombinant adenovirus expressing IL-24 could enhance the sensitivity of lung cancer cells to chemotherapeutic drugs DDP.
关键词
腺病毒/人白细胞介素-24/肺癌/基因治疗/顺铂Key words
adenovirus/IL-24/pulmonary carcinoma/gene therapy/cisplatin引用本文复制引用
基金项目
江苏省“六大人才高峰”第八批资助项目计划(056)
出版年
2012
NETL
NSTL
维普
万方数据