非小细胞肺癌中EphB 4的表达及基因多态性
Genetic polymorphism and protein expression of EphB 4 in non-small cell lung cancer
纪勇 1陈静瑜 1郑明峰 1吴小波 1叶书高 1陆荣国1
作者信息
- 1. 无锡市人民医院胸外科,江苏无锡214023
- 折叠
摘要
目的 观察EphB 4在非小细胞肺癌(NSCLC)中的蛋白表达和基因多态性情况,探讨EphB 4与NSCLC的关系.方法 采用免疫组织化学染色方法和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分别检测28例NSCLC患者癌组织和外周血中EphB 4蛋白表达和rs314310(C/A)基因多态性情况,并与12例癌旁正常肺组织和30名正常健康人外周血作对比,分析EphB 4的蛋白表达和基因多态性与NSCLC的关系.结果 NSCLC组织中EphB 4的阳性率(53.6％)明显高于癌旁正常肺组织(0.0％),(P＜0.05)；其EphB4表达与分化程度、淋巴结转移和TNM分期有关(P＜0.05或＜0.01),而与性别、年龄和组织来源没有关系(均P＞0.05).NSCLC患者和正常健康人群外周血中rs314310的CC、CA、AA基因型频率分别为25.0％、53.6％、21.4％和43.3％、53.3％、3.3％,差异均无统计学意义(均P＞0.05)；C和A等位基因频率分别为51.8％、48.2％和70.0％、30.0％,差异均有统计学意义(均P＜0.05).进一步分析显示,AA基因型患者的EphB 4的阳性率明显高于其他基因型患者(P＜0.05).结论高表达的EphB 4在NSCLC的发生发展中有重要的作用,其rs314310AA基因型可能是NSCLC的危险因素.
Abstract
Objective To investigate the genetic polymorphism distribution and protein expression of EphB 4 in non-small cell lung cancer (NSCLC ) and to study the possible relationship between EphB 4 and NSCLC. Methods Immunohistochemical method was used to detect the expression level of EphB 4 in 28 tumor tissues. Polymerase chain reaction-restriction fragment length polymorphism PCR ( PCR-RFLP)was used for the detection of polymorphism of EphB 4 gene in peripheral blood DNA from 28 NSCLC patients and 30 normal samples. Results EphB 4 gene in cancer tissues was significantly higher than the level of normal organization (53.6% vs 0.0% , P <0. 05). The expression level of EphB 4 protein in lung cancer tissues had no significant correlations with the sex,age of the patients, source of the primary cancer(P >0.05), but associated with TNM stages, histological classification, P-TNM status(P <0.05). The frequencies of rs314310 genotypes CC, CA, AA were 25.0% ,53.6% ,21.4% in NSCLC group and 43. 3% ,53.3% ,3. 3% in control group respectively. No significant difference was found between the two groups (P >0.05). The frequencies of EphB 4 C and A allele were 51. 8% ,48.2%in control group and 70.0% ,30.0%in lung cancer group respectively. The expression of EphB 4 of AA genotypes was significantly higher than other genotypes (P <0.05). Conclusion Higher expression of EphB 4 gene associated with the occurrence and development of NSCLC. rs314310 AA genotype might be a risk factor on occurrence of NSCLC.
关键词
非小细胞肺癌/EphB/4/免疫组织化学/PCR-RFLP/单核苷酸多态性Key words
non-small cell lung cancer/EphB 4/polymerase chain reaction-restriction fragment length polymorphism/single nucleotide polymorphism/immunohistochemistry引用本文复制引用
基金项目
江苏省重大科技与自主创新基金资助项目(BE20101016)
出版年
2012
NETL
NSTL
万方数据