Objective To research the potentiate antitumor and suppress acute nephrotoxicity effects of acanthopanax extract on cantharidin in Aidi injection. Methods MTT essay was used to observe the effect of acanthopanax extract on cytotoxicity caused by cantharidin on cells (SMMC-7721 human hepato-cellular cells and NRK rat kidney cells). Flow cytometry was used to analyze the apoptosis rate of SMMC-7721 and NRK cells. The protective effects of acanthopanax extract on renal injury caused by cantharidin in rats were projected. Results Acanthopanax extract could not only enhance inhibitional proliferation induced by cantharidin on SMMC-7721 cells, but also reduce cytotoxicity caused by cantharidin on NRK cells. Meanwhile the results of flow cytometry showed that acanthopanax extract could improve the apoptosis rate induced by cantharidin on SMMC-7721 cells, as well as reduce the apoptosis rate on NRK cells. Acanthopanax extract could decrease the contents of blood urea nitrogen, serum creatinine and proteinuria induced by cantharidin in rats. Conclusion Acanthopanax extract has markly effect enhancing and toxic-ity reducing effect on cantharidin in Aidi injection.
Aidi injectionacanthopanax extractcantharidinpotentiate antitumor and toxicity reducing
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