Objective To investigate improved method of rat subarachnoid catheterization, fixing and protecting. Methods Twenty-four adult male SD rats were randomly divided into 2 groups ( n = 12), the Yaksh group (group Y) and the experiment group (group E). The traditional Yaksh method was used in the group Y to place intrathecal catheter with no mental shielding protection. Improved in-trathecal catheter placement with mental shielding protection was used in the group E. The rat' s paw withdrawal latency to thermal stimuli (PWTL), paw withdrawal mechanical threshold (PWMT), hindpaw withdrawal latency (HWL) was measured in the study. Results Rat's behavior data showed no difference in the baseline threshold. The PWMT value in day 1 and 4 in the group Y decreased more than that in the group E( P < 0.05). The HWL value decreased more in dayl and 4 in the group Y that that in the E group(p <0.05). Olny 50% catheter still existed in the rats in the group Y without mental shielding protection 1 day after being catheterlizated. Twenty-five Percent catheter was able to be used in day 4 after intrathecal catheter was used and only 16.7% catheters were used for more than 7 days. Conversely, the external portions of the catheters were kept intact in all rats with mental shielding protect at 21 days after the surgery in the group E. Conclusion Through improving the way of place intrathecal catheter and metal cover use, rats have no obvious behavior change. Meanwhile, the number of experiment rats consumption and catheter destroy rate significantly reduce.
NETL
NSTL
维普
万方数据
