大鼠小脑间位核注射氨己烯酸对淋巴细胞功能的影响
Effect of vigabatrin injection in the cerebellar interposed nuclei on lymphocyte function
陆健花 1徐芬芬 1曹蓓蓓 1彭聿平 1邱一华1
作者信息
- 1. 南通大学医学院生理学系,江苏南通226001
- 折叠
摘要
目的 探讨小脑间位核GABA能系统对免疫功能的调节.方法 大鼠小脑间位核内微量注射GABA转氨酶抑制剂氨己烯酸(VGB),瑞氏染色法检测外周血白细胞中淋巴细胞百分比的变化；四甲基偶氮唑(MTT)比色法测定淋巴细胞的增殖功能；绵羊红细胞腹腔免疫大鼠,酶联免疫吸附测定法(ELISA)检测血清中抗绵羊红细胞IgM、IsG抗体的水平.结果 大鼠双侧小脑间位核微量注射VGB后,与对照组相比,外周血白细胞中淋巴细胞百分比、T淋巴细胞对刀豆蛋白A诱导的增殖能力和血清中抗绵羊红细胞IgM、IgG抗体的水平均明显降低,而两对照组间(未处理对照组和生理盐水对照组)各项指标的差异均无统计学意义(均P ＞0.05).结论 大鼠小脑间位核GABA能系统参与对免疫功能的调节.
Abstract
Objective To explore the effect of cerebellar interposed nuclei (IN) GABAergic system on immunomodulation. Methods GABA transaminase inhibitor, vigabatrin (VGB) was microinjected into the bilateral IN. On the third day following the injection, lymphocyte percentage in the peripheral blood leukocyte was measured by Wright's stain, lymphocyte proliferative response to concanavalin A (Con A) was examined by colorimetric assay of methyl thiazole tetrazolium ( MTT) , and anti-sheep red blood cell ( SRBC ) IgM and IgG antibodies in the serum were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with intact or saline-injected rats, VGB injection in bilateral cerebellar IN led to a decrease in lymphocyte percentage in peripheral white blood cells, a suppression of T cell proliferative response to Con A, and a diminishment of serum specific anti-SRBC IgM and IgG antibodies, there was no significant difference between the two groups of control rats. Conclusion Cerebellar IN GABAergic system is actively implicated in regulation of immune function.
关键词
小脑/间位核/氨己烯酸/淋巴细胞/免疫功能/大鼠Key words
cerebellum/interposed nucleus/vigabatrin/lymphocyte/immune function/rat引用本文复制引用
基金项目
国家自然科学基金(30870819)
江苏省自然科学基金资助项目(BK2010278)
出版年
2012
NETL
NSTL
维普
万方数据