Objective To induce human embryonic germ cell ( hEGC) differentiation into cardiomyocytes, find out its process and regulation mechanism preliminary. Methods The tissue was cultured in vitro to obtain hEGC. hEGCs were induced to differentiate into cardiocytes by ascorbic acid. Cell proliferation rate was detected by the CCK-8. Cx43 was detected by immunofluorescence on differentiated cells. Quantitative RT-PCR was applied to evaluate GATA-4, ACTC1 and other relevant gene expression by HEGC induced myocardid cell. Bioinformatics was used to analyze the interaction relationship between the proteins relate to the process of differentiation. Results Cx43 expression was positive in differentiated cells, showing their proliferation ability to a certain degree; the expression strength of GATA-4, ACTC1 is different from the hEGC before differentiation; there is intimate relationship among GATA-4,Nkx2. 5 and the cardiac troponin. Conclusion hEGCs could be induced to differentiate into cardiomyocytes. Proteomics method is qualified to assist us in researching the differentiation process and control mechanism.
human embryonic germ cellcell differentiationbioinformaticsRT-PCR
NETL
NSTL
维普
万方数据
