运动对成年小鼠肌肉氧自由基和自噬水平的影响
Study on the oxyradical and autophagy level of muscle in the adult exercising mice
卞龙艳 1蒋丹 2林芳2
作者信息
- 1. 盐城卫生职业技术学院护理系外科护理教研室,江苏盐城224006
- 2. 苏州大学医学部药学院衰老与神经疾病实验室,江苏苏州215123
- 折叠
摘要
目的 研究运动对成年小鼠肌肉氧自由基和自噬水平的影响.方法 将实验组和对照组小鼠自由运动2个月后处死,取骨骼肌、心肌.检测骨骼肌SOD、MDA水平以观察氧自由基的水平；采用Western blot技术检测骨骼肌、心肌LC3、p62蛋白水平以观察自噬水平的变化.结果 实验组小鼠肌肉中的SOD水平显著高于对照组,而MDA水平显著低于对照组(均P＜0.01)；实验组小鼠骨骼肌中LC3-Ⅱ/LC3-Ⅰ比对照组高约14％,p62比对照组低约40％；实验组小鼠心肌LC3-Ⅱ/LC3-Ⅰ比对照组高约20％,p62比对照组低约30％,差异均有统计学意义(均P＜0.05).结论 运动能够提高骨骼肌的抗氧化酶的活性,激活骨骼肌和心肌的自噬,提示运动抗衰老与自噬的激活有关.
Abstract
Objective To study the exercise effects on oxyradical and autophagy levels of muscle in the adult mouse. Methods Take skeletal muscles and myocardia from two groups of mice ( experimental group and control group) that exercised for two months and be killed. Detect the skeletal muscle' s SOD and MDA levels so as to observe the oxyradical levels; Using Western blot to detect the levels of skeletal muscle, myocardial LC3, p62 protein, so as to observe the changes of autophagy level. Results The mouse muscle' s SOD level of experimental group was significantly higher than that in the control group, but MDA level was significantly lower than the control group (all P<0.01) ; the skeletal muscle LC3-H/ LC3- I in experimental group was higher than the control group of about 14% , p62 was lower than the control group of about 40% ; the myocardial LC3-II/ LC3- I of experimental was higher than the control group of about 20% , p62 was significantly lower about 30% (all P <0.05). Conclusion Exercise could improve skeletal muscle antioxidant enzyme activity, activation of skeletal muscle and cardiac autophagy, tips for moving anti-aging related to autophagy activation.
关键词
运动/肌肉/自噬/氧自由基Key words
exercise/muscle/autophagy/oxyradical引用本文复制引用
基金项目
盐城市卫生局资助项目(YK2011126)
盐城卫生职业技术学院青年基金资助项目()
出版年
2012
NETL
NSTL
维普
万方数据