晚期糖基化终末产物对人骨髓间充质干细胞体外增殖的影响
Effect of advanced glycosylation end products (AGEs) on proliferation of human bone marrow mesenchymal stem cells (MSCs) in vitro
陆轶群 1鲁燕 1李慧娟 1成兴波1
作者信息
- 1. 苏州大学附属第一医院内分泌科,江苏苏州215006
- 折叠
摘要
目的 研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响.方法 采用CCK-8比色法检测在不同时间和浓度AGEs干预下所培养的MSCs的吸光度和增殖活力,同时测定细胞内的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.结果 AGEs加入细胞培养后,可显著抑制MSCs的增殖(均P＜0.01),并呈剂量和时间依赖效应关系.随着AGEs-BSA作用浓度的增加,细胞内丙二醛含量明显增加,而细胞匀浆中超氧化物歧化酶的活性却受到了抑制(均P＜0.01),具有剂量依赖效应.结论 AGEs通过增强MSCs内的氧化应激,破坏MSCs内环境稳定性,从而抑制MSCs的增殖.研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响.
Abstract
Objective To explore the effect of advanced glycosylation end products ( AGEs) on proliferation of human bone marrow mesenchymal stem cells (MSCs) in vitro and relevent mechanism. Methods Absorbance was assayed using CCK-8 colorimetry to analysis the proliferation activity of MSCs under the intervention of AGEs, the content of MDA and the activity of SOD were measured. Results The proliferation activity of MSCs was significantly inhibited when AGEs were added to culture medium, and the effects were dose-depedent and time-dependent. Compared with control groups, co-culturing with AGEs-BSA increased the content of MDA in cells, and simultaneously the activiey of SOD was decreased, both in a dose dependent manner. Conclusion AGEs breaks the homeostasis of cell by increasing the oxidative stress, and accordingly, inhibit the proliferation of MSCs in vitro significantly.
关键词
糖基化终末产物/骨髓间充质干细胞/细胞增殖/氧化应激Key words
advanced glycosylation end products/bone marrow mesenchymal stem cells/cell prolif-eration/oxidative stress引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据