Objective To explore the effect of advanced glycosylation end products ( AGEs) on proliferation of human bone marrow mesenchymal stem cells (MSCs) in vitro and relevent mechanism. Methods Absorbance was assayed using CCK-8 colorimetry to analysis the proliferation activity of MSCs under the intervention of AGEs, the content of MDA and the activity of SOD were measured. Results The proliferation activity of MSCs was significantly inhibited when AGEs were added to culture medium, and the effects were dose-depedent and time-dependent. Compared with control groups, co-culturing with AGEs-BSA increased the content of MDA in cells, and simultaneously the activiey of SOD was decreased, both in a dose dependent manner. Conclusion AGEs breaks the homeostasis of cell by increasing the oxidative stress, and accordingly, inhibit the proliferation of MSCs in vitro significantly.
advanced glycosylation end productsbone marrow mesenchymal stem cellscell prolif-erationoxidative stress
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