Objective To investigate the expressions of COX-2, Bcl-2 and Bax mRNA in human breast cancer SK-BR-3 cells after incubation with UA and to dedect the expression of apoptosis-related proteins in the tissue of breast cancer, and to provide a reliable experimental basis for clinical applicaton of UA. Methods The expressions of COX-2, Bcl-2 and Bax mRNA in SK-BR-3 cells were determined by RT-PCR;The expressions of COX-2,Bcl-2 and Bax protein in estrogen receptor(ER)-negative and human epidermal growth-factor 2 ( HER2 ) positivity human breast cancer were determined by immunohistochemistry. Results The expression of COX-2 and Bcl-2 mRNA of SK-BR-3 cells treated by UA for 48 h decreased ( P < 0. 05 ) , Bcl-2 / Bax reduced ( P < 0. 05 ) , whereas the expression of Bax mRNA unchanged; The positivvity rate of COX-2 , Bcl-2 . Bax protein in estrogen receptor-negative and human epidermal growth-factor 2 positive breast cancer were 86. 67% (26/30) , 63. 33% (19/30) and 56. 67% (17/30) Respectively. The expression of COX-2 and Bax was negatively correlated(P <0. 05) , whereas the expression of COX-2 and Bcl-2 was no significant correlation. Conclusion UA inhibites COX-2 and Bcl-2 mRNA expression and reduces the ratio of Bcl-2 / Bax; There is a higher positive expression of COX-2 in estrogen receptor-negative and human epidermal growth-factor 2 positive human breast cancer;The expression of COX-2 and Bax is negatively correlated, whereas the expression of COX-2 and Bcl-2 is-no significant correlation.
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NSTL
维普
万方数据
