乳腺癌中COX-2、Bax、Bcl-2的表达及熊果酸的干预作用
The expressions of COX-2, Bax and Bcl-2 in the tissues of breast cancer and intervention effect of ursolic acid
徐新伟 1郭玲玲 2顾振纶 3蒋小岗 3周文轩 3郭次仪3
作者信息
- 1. 苏州中药研究所,江苏苏州215007;苏州大学医学部病理学与病理生理学系,江苏苏州215123;杭州市余杭区第一人民医院病理科,浙江杭州311100
- 2. 苏州大学医学部病理学与病理生理学系,江苏苏州215123
- 3. 苏州中药研究所,江苏苏州215007
- 折叠
摘要
目的 研究熊果酸(UA)对人乳腺癌SK-BR-3细胞COX-2、Bax、Bcl-2 mRNA的表达的影响,结合人体乳腺癌组织凋亡相关蛋白表达检测,探讨其作用机制,为UA的临床应用提供实验依据.方法 RT-PCR技术检测SK-BR-3细胞中COX-2、Bax、Bcl-2 mRNA的表达；免疫组织化学技术检测雌激素受体(ER)(-),人表皮生长因子受体-2(HER-2)(+)的人乳腺癌组织中COX-2、Bax、Bcl-2蛋白的表达.结果 UA作用48 h后能使乳腺癌SK-BR-3细胞中COX-2、Bcl-2 mRNA表达明显减少(P＜0.05),使Bcl-2/Bax比值减小(P＜0.05),而对Bax mRNA的表达未见明显影响；COX-2、Bcl-2、Bax蛋白在ER(-),HER-2(+)的乳腺癌组织中的表达阳性率分别为86.67％ (26/30),63.33％(19/30),56.67％(17/30),COX-2与Bax表达呈明显负相关(P＜0.05),与Bcl-2未见明显相关性.结论 UA可抑制COX-2、Bcl-2 mRNA表达,使Bcl-2/Bax减小；ER(-),HER-2(+)的人乳腺癌组织中COX-2中阳性表达率较高,COX-2与Bax表达呈明显负相关,与Bcl-2表达未见明显相关性.
Abstract
Objective To investigate the expressions of COX-2, Bcl-2 and Bax mRNA in human breast cancer SK-BR-3 cells after incubation with UA and to dedect the expression of apoptosis-related proteins in the tissue of breast cancer, and to provide a reliable experimental basis for clinical applicaton of UA. Methods The expressions of COX-2, Bcl-2 and Bax mRNA in SK-BR-3 cells were determined by RT-PCR;The expressions of COX-2,Bcl-2 and Bax protein in estrogen receptor(ER)-negative and human epidermal growth-factor 2 ( HER2 ) positivity human breast cancer were determined by immunohistochemistry. Results The expression of COX-2 and Bcl-2 mRNA of SK-BR-3 cells treated by UA for 48 h decreased ( P < 0. 05 ) , Bcl-2 / Bax reduced ( P < 0. 05 ) , whereas the expression of Bax mRNA unchanged; The positivvity rate of COX-2 , Bcl-2 . Bax protein in estrogen receptor-negative and human epidermal growth-factor 2 positive breast cancer were 86. 67% (26/30) , 63. 33% (19/30) and 56. 67% (17/30) Respectively. The expression of COX-2 and Bax was negatively correlated(P <0. 05) , whereas the expression of COX-2 and Bcl-2 was no significant correlation. Conclusion UA inhibites COX-2 and Bcl-2 mRNA expression and reduces the ratio of Bcl-2 / Bax; There is a higher positive expression of COX-2 in estrogen receptor-negative and human epidermal growth-factor 2 positive human breast cancer;The expression of COX-2 and Bax is negatively correlated, whereas the expression of COX-2 and Bcl-2 is-no significant correlation.
关键词
熊果酸/人表皮生长因子受体2/COX-2/Bax/Bcl-2/SK-BR-3细胞Key words
ursolic acid/human epidermal growth factor receptor-2/COX-2/Bax/Bcl-2/SK-BR-3 cells引用本文复制引用
基金项目
香港保健协会科研基金资助项目(20060909-5HK)
出版年
2012
NETL
NSTL
维普
万方数据