亲水作用色谱法测定猫豆中左旋多巴及其衍生物的含量
Determination of L-dopa and its derivate in seeds of Mucuna pruriens var.utilis by hydrophilic chromatography
谭蓓蓓 1俞樱露 1李楠 1刘江云 1李贺然 1蔡培烈 1杨世林1
作者信息
- 1. 苏州大学医学部药学院,江苏苏州215123
- 折叠
摘要
目的 建立测定猫豆中左旋多巴及其衍生物1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(MCD)含量的亲水作用高效液相色谱法.方法 采用Cosmosil HILIC色谱柱(4.6 mm×250 mm,5μm),柱温30℃,以乙腈-(10 mmol/L)乙酸铵(80∶20)为流动相,体积流量1.0 ml/min,检测波长280 nm.结果 MCD及左旋多巴在0.081 36～2.034 μg和0.126 9 ～3.172 μg范围内线性关系良好,平均回收率(n=6)分别为98.11％和99.86％,相对标准偏差(RSD)分别为3.32％和0.82％.结论 该方法简便、准确,可用于定量分析猫豆中的左旋多巴及MCD.
Abstract
Objective A hydrophilic interaction liquid chromatography method was developed for quantitative determination of L-dopa and 3-Carboxy-l-methyl-6,7-dibydroxy-1,2,3,4-tetrahydroisoquino-line (MCD) in seeds of Mucuna pruriens var. utilis Burck. Method Chromatographic separation was performed on a Cosmosil HILIC analytical column (4.6 mm × 250 mm, 5 μm) at 30 ℃ with acetonitrile -(10 mmol/L) ammonium acetate solution (80:20)as the mobile phase. The detection wavelength was at 280 nm and the flow rate was 1. 0 ml/min. Results The linear ranges were 0. 08136 - 2. 034 μg for MCD, 0.1269 -3.172 μg for L-dopa; the average recoveries(n =6) of two compounds were 98.11 % ( RSD =3.32 % ) and 99. 86 % ( RSD = 0. 82 % ) respectively. Conclusion A simple and accurate HPLC method was developed for the quantitative determination of L-dopa and MCD in seeds of Mucuna pruriens var. utilis.
关键词
猫豆/亲水作用色谱法/左旋多巴/1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉Key words
Mucuna pruriens var. utilis/hydrophilic interaction liquid chromatpgraphy/L-dopa/3-Carboxy-1 -methyl-6,7-dihydroxy-1 ,2,3,4-tetrahydroisoquinoline引用本文复制引用
基金项目
国家自然科学基金(81274190)
江苏高校优势学科建设工程资助项目(PAPD)()
出版年
2012
NETL
NSTL
维普
万方数据