首页|亲水作用色谱法测定猫豆中左旋多巴及其衍生物的含量

亲水作用色谱法测定猫豆中左旋多巴及其衍生物的含量

Determination of L-dopa and its derivate in seeds of Mucuna pruriens var.utilis by hydrophilic chromatography

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目的 建立测定猫豆中左旋多巴及其衍生物1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(MCD)含量的亲水作用高效液相色谱法.方法 采用Cosmosil HILIC色谱柱(4.6 mm×250 mm,5μm),柱温30℃,以乙腈-(10 mmol/L)乙酸铵(80∶20)为流动相,体积流量1.0 ml/min,检测波长280 nm.结果 MCD及左旋多巴在0.081 36～2.034 μg和0.126 9 ～3.172 μg范围内线性关系良好,平均回收率(n=6)分别为98.11％和99.86％,相对标准偏差(RSD)分别为3.32％和0.82％.结论 该方法简便、准确,可用于定量分析猫豆中的左旋多巴及MCD.
Objective A hydrophilic interaction liquid chromatography method was developed for quantitative determination of L-dopa and 3-Carboxy-l-methyl-6,7-dibydroxy-1,2,3,4-tetrahydroisoquino-line (MCD) in seeds of Mucuna pruriens var. utilis Burck. Method Chromatographic separation was performed on a Cosmosil HILIC analytical column (4.6 mm × 250 mm, 5 μm) at 30 ℃ with acetonitrile -(10 mmol/L) ammonium acetate solution (80:20)as the mobile phase. The detection wavelength was at 280 nm and the flow rate was 1. 0 ml/min. Results The linear ranges were 0. 08136 - 2. 034 μg for MCD, 0.1269 -3.172 μg for L-dopa; the average recoveries(n =6) of two compounds were 98.11 % ( RSD =3.32 % ) and 99. 86 % ( RSD = 0. 82 % ) respectively. Conclusion A simple and accurate HPLC method was developed for the quantitative determination of L-dopa and MCD in seeds of Mucuna pruriens var. utilis.

Mucuna pruriens var. utilishydrophilic interaction liquid chromatpgraphyL-dopa3-Carboxy-1 -methyl-6,7-dihydroxy-1 ,2,3,4-tetrahydroisoquinoline

谭蓓蓓、俞樱露、李楠、刘江云、李贺然、蔡培烈、杨世林

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苏州大学医学部药学院,江苏苏州215123

猫豆 亲水作用色谱法 左旋多巴 1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉

国家自然科学基金江苏高校优势学科建设工程资助项目(PAPD)

81274190

2012

苏州大学学报(医学版)
苏州大学

苏州大学学报(医学版)

CSTPCD
影响因子：0.499
ISSN：1673-0399
年,卷(期)：2012.32(5)
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