Objective To study the antioxidant activities of succinated glucomannan from Bletilla striata. Methods Antioxidant activities of succinated glucomannan with different degree of dubstitution (DS) including BT-2 (DS =0. 38) , BT-3 (DS =0. 69) and BT4 (DS =0. 85) against OH ·and O2- · were measured by Fenton reaction and autoxibation of pyrogallic acid. Results The IC50 ( mg/ml) of succinated glucomannans against OH · was measured to be 1.48 ±0.01 (BT-4) , 2.65 ±0.01 (BT-3), 2.79 ±0.02 (BT-2); while their IC50(mg/ml) against O2- · was determined to be 1. 38 ±0. 06 (BT-4), 6.44 ±0.02 (BT-3), 21.33 ±0.13 (BT-2), respectively. Conclusion The antioxidant activities of succinated glucomannans against OH · and O2- · were increased apparently with increasing DS.
Bletilla striatasuccinated glucomannandegrees of substitutionantioxidant activities
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