Objective To set up a method for determination of molecular weight and distribution of dextran 40 by high performance size exclusion chromatography ( HPSEC) with evaporative light scattering detector (ELSD). Method The TSK-Gel G4000 PWXL (. 10 μm, 7. 8 mm × 300 mm ) column was applied with water as mobile phase. The flow rate was 0. 6 ml/min, and column temperature was set at 30℃. Results As for the average molecular weight of dextran, the linear range was between 4 600 ~ 133 800 u(R2 =0. 991). Relative standard deviations (RSD) of precision and repeatability were 0.252% and 0. 460% respectively. Conclusion The method is accurate with high sensitivity which could be applied for the measurement and quality control of molecular weight and distribution of dextran 40.
dextranmolecular weighthigh performance gel permeation chromatographyevaporative light scattering detector
NETL
NSTL
维普
万方数据
