沙门菌质粒对细菌生物膜形式的影响
The effect of Salmonella typhi plasmid on biofilm formation
廖莉 1吴淑燕 1李嫄渊 1刘珍 1黄瑞1
作者信息
- 1. 苏州大学医学部病原生物学系,江苏苏州215123
- 折叠
摘要
目的 研究伤寒沙门菌质粒pRST98对生物膜形成的影响.方法 将携带pRST98的野生型伤寒沙门菌于30℃和37℃培养3d,用半定量法确定生物膜形成的适宜温度.将稀释的菌液于30℃分别培养12 h、1d、2d、3d、4d和5d,用半定量法确定生物膜成熟的时间.分别用携带pRST98的野生型伤寒沙门菌,消除pRST98的突变株及pRST98的回补株建立生物膜模型,用结晶紫染色法,半定量法和扫描电镜观察三种株受试菌形成生物膜的能力.结果 在30℃培养时细菌生物膜形成能力高于37℃,此温度为沙门菌生物膜形成的适宜温度；3d时生物膜趋于成熟；野生株和回补株形成生物膜的能力显著高于突变株.结论 伤寒沙门菌质粒pRST98与该菌生物膜形成密切相关.
Abstract
Objective To investigate the effect of Salmonella typhi(S. typhi) plasmid on the formation of biofilm. Methods Wild-type S. typhi strain carrying plasmid pRST98 was cultured at 301 or 371 for 3 d to determine the optimum temperature of biofilm formation using semi-quantitative method. Then the strain was cultured at 30℃ for 12h, 1d,2d,3d,4d and 5 d to determine the biofilm mature time using semi-quantitative method. Wild-type S. typhi strain carrying plasmid pRST98, pRST98-deletion S. typhi strain and pRST98-complemented S. typhi strain were used and then the biofilm formation ability of these three strains was compared by using crystal violet staining method, semi-quantitative method and scanning electron micrographs. Results The ability of biofilm formation was better at 30℃ , which is the optimum temperature of Salmonella biofilm formation. Biofilm became mature after cultured for 3 d. The ability biofilm formation by wild-type and pRST98-complemented 5. typhi strain to form biofilm was stronger than that of pRST98-deletion S. typhi strain. Conclusion Plasmid pRST98 is closely related to biofilm formation of S. typhi.
关键词
伤寒沙门菌/质粒/生物膜Key words
Salmonella typhi/plasmid/biofilm引用本文复制引用
基金项目
国家自然科学基金(30972768)
江苏省自然科学基金(BK2011286)
教育部高等学校博士学科点专项科研基金(2010320111009)
出版年
2012
NETL
NSTL
维普
万方数据