IRM-2小鼠cDNA文库的全长cDNA序列分析
Full-length cDNA sequence analysis on IRM-2 mouse cDNA library
王芹 1刘晓秋 1宋力 1孙志娟 1杜利清 1徐畅 1王彦 1刘强 1李进 1樊飞跃1
作者信息
- 1. 北京协和医学院,中国医学科学院放射医学研究所生物室,天津市分子核医学重点实验室,天津300192
- 折叠
摘要
目的 分析IRM-2小鼠全长cDNA文库的cDNA序列.方法 根据IRM-2小鼠的21条EST,从IRM-2小鼠全长cDNA文库测定基于该21条EST序列PCR扩增的全长cDNA序列,通过与GenBank基因库同源序列信息比对,分析全长cDNA序列和性质.结果 根据21对引物PCR产物的拼接,获得5条全长cDNA序列,与小鼠已知基因不同源,而且得到了可能编码蛋白质的氨基酸序列和蛋白质的性质.结论 5条全长cDNA序列提示IRM-2小鼠体内可能存在辐射抗性相关基因,为进一步分离和鉴定辐射抗性相关基因奠定了基础.
Abstract
Objective To analyse cDNA sequences from full-length cDNA library of IRM-2 mice. Methods Full-length cDNA products were amplified by PCR from cDNA library of IRM-2 mice according to twenty-one pieces of EST. Full-length cDNA sequences and properties were analyzed by comparing with GenBank database. Results Five pieces of full-length cDNA were obtained according to PCR products amplified by twenty-one pairs of primers, which were not the same as that of known mice genes. A-mino acid sequences and properties of proteins encoded by five pieces of full-length cDNA were acquired. Conclusion Five pieces of full-length cDNA imply that radioresistance correlative genes may be existed in IRM-2 mice, laying a foundation for isolating and identifying radioresistance correlative genes in further study.
关键词
IRM-2小鼠/cDNA文库/cDNA序列/氨基酸序列Key words
IRM-2 mice/cDNA library/cDNA sequence/amino acid sequence引用本文复制引用
基金项目
国家自然科学基金(30870583)
中国医学科学院放射医学研究所学科发展基金资助项目(SF0822)
出版年
2012
NETL
NSTL
维普
万方数据