共培养体系中TGF-β1沉默大肠癌LoVo细胞对巨噬细胞IL-12和TNF-α分泌的影响
Effects of TGF-β1 silencing of colon cancer LoVo cell on IL-12 and TNF-α secretion by the co-cultured macrophages
顾新华 1周辰超 2谭纪伏 1袁政 1柳燕东 2罗家乐 2季清华 2王益 2陈城 2高蓓 2王守立2
作者信息
- 1. 苏州市立医院普外科,江苏苏州215002
- 2. 苏州大学医学部病理学系,江苏苏州215123
- 折叠
摘要
目的 研究TGF-β1沉默人大肠癌LoVo细胞对人巨噬细胞系28SC免疫功能的影响.方法 将构建的TGF-β1siRNA稳定表达LoVo细胞与人巨噬细胞系28SC共培养,酶联免疫吸附实验检测培养液中IL-12和TNF-α水平,同时设立对照组空载体pSUPER-neo-gfp稳定转染LoVo细胞+28SC.结果 TGF-β1siRNALoVo+ 28SC组IL-12和TNF-α分泌量高于对照组(均P＜0.05)；TGF-β1+28SC组IL-12和TNF-α分泌量与LoVo +28SC组之间无明显差异(P＞0.05).结论 大肠癌LoVo细胞分泌的TGF-β1能通过影响IL-12和TNF-α的分泌抑制巨噬细胞的免疫功能,有望通过阻断TGF-β1信号转导改善肿瘤免疫微环境抑制大肠癌的发生发展.
Abstract
Objective To study the effects of TGF-pl silencing of human colon cancer LoVo cell on immune function of human macrophages cell line 28 SC. Methods LoVo cells stably expressing TGF-β1 siRNA were cultivated together with human macrophages cell line 28SC. The levels of IL-12 and TNF-a in culture medium were detected by enzyme linked immunosorbent assay (ELISA). While a control group was established. Results The concentrations of IL-12 and TNF-a in TGF-β1 siRNA LoVo +28SC group were higher than those in control group (P <0.05). The concentrations of IL-12 and TNF-a in TGF-β1 + 28SC group and control group had no significant difference (P>0.05). Conclusion TGF-pl secreted by colon cancer LoVo cells can inhibit the immune function of macrophages through affecting the secretion of IL-12 and TNF-α. And it is hopeful to inhibit the development of colon cancer through blocking TGF-pl signal transduction.
关键词
转化生长因子β1/大肠癌/肿瘤微环境Key words
transforming growth factor β1/colon cancer/tumor mieroenvironment引用本文复制引用
基金项目
国家自然科学基金(81272738)
苏科技[2011]231号(SYSD2011112)
苏州大学大学生课外学术科研基金(KY2012101A)
苏州大学大学生创新实验计划(5731515611)
出版年
2012
NETL
NSTL
维普
万方数据