Objective To study the effects of TGF-pl silencing of human colon cancer LoVo cell on immune function of human macrophages cell line 28 SC. Methods LoVo cells stably expressing TGF-β1 siRNA were cultivated together with human macrophages cell line 28SC. The levels of IL-12 and TNF-a in culture medium were detected by enzyme linked immunosorbent assay (ELISA). While a control group was established. Results The concentrations of IL-12 and TNF-a in TGF-β1 siRNA LoVo +28SC group were higher than those in control group (P <0.05). The concentrations of IL-12 and TNF-a in TGF-β1 + 28SC group and control group had no significant difference (P>0.05). Conclusion TGF-pl secreted by colon cancer LoVo cells can inhibit the immune function of macrophages through affecting the secretion of IL-12 and TNF-α. And it is hopeful to inhibit the development of colon cancer through blocking TGF-pl signal transduction.
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