Objective To investigate the effect of eEF-2 kinase inhibitor on proliferation of glioma cell line LN229. Method After eEF-2 kinase was inhibited, proliferation and viability of glioma LN229 cell was determined by MTT and trypan blue assay respectively. Morphological changes of tumor cells were detected by light microscope. Cell cycle was analyzed by flow cytometry. The levels of phospho-eEF2 , Cleaved Casepase-3 and PARP proteins were measured by Western blot analysis. Results Following the inhibition of eEF-2 kinase, the proliferation of LN229 giloma cells was significantly inhibited in a time-concentration dependent manner (P <0.05 or 0.01). Twenty-four hours after treatment, along with the increasing concentrations of NH125, LN229 cell viability significantly decreased (P < 0.05 or 0.01) , and cell morphology altered. After being treated with NH125(0.5 μmol/L) for 24 h, LN229 giloma cell cycle was arrested at S phase and with a small apoptotic peak, the expression of phospho-eEF2 (Thr56) was decreased, and the expressions of Cleaved Caspase-3, Cleaved PARP were increased. Conclusion Inhibition of eEF-2 kinase caused a significant suppression of proliferation and a significant reduction of the cell viability of human glioma LN229 cells. The underlying mechanisms may be related to induction of cell cycle arrest and the activation of Casepase-3 and PARP-related apoptosis pathway.
eEF-2 kinasehuman glioma cell line LN229NH125cell proliferation
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NSTL
维普
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