Objective To investigate the method of Salmonella spp. mutant construction, optimizes the processing and provides the basis for further experiments in genetically modification. Methods Two different plasmid vectors DNAs pMDT-GFP and pGBM151-spvB were electrotransformed into S. typhimuri-um under various conditions including growth stage, medium, concentration of competent cells and pulse strength. The transformation efficiency changes were discovered and the optimized conditions were evaluated accordingly. Results The optimal electrotransformation effect can be obtained when A600 is 0. 3 , the medium is hypertonic LB contained 15g/L NaCl, concentration of competent cells is 1010 cfu/ml and the electroporation parameters is 2.5 kV, 400 Ω,50 μF. Conclusion This method can be used to efficiently of construct Salmonella spp. mutant.
NETL
NSTL
维普
万方数据
