Objective To investigate the effects of naloxone on hypoxia-induced rat neuron injury. Methods Rat neuron were cultured and divided into control group, hypoxia-induced group, naloxone pretreated group. Neuron apoptosis was detected by using flow cytometry. Fluorescent probe, 2,7-dichloro-fluorescein diacetate ( DCFDA) , was used to examine intracellular reactive oxygen species ( ROS) generation. Superoxide dismutase ( SOD) activity was accessed spectrophotometrically by using SOD assay kit. NF-κB activation was detected by western blotting. Results (1 ) Naloxone inhibited hypoxia-induced rat neuron apoptosis. Hypoxic rats neuronal apoptosis significantly increased 3.54 times than that of the control group (all P <0. 05) . (2) Naloxone inhibited hypoxia-induced ROS generation. Hypoxia-induced neuron reactive oxygen species generation, was 2. 66 times than that of the control group, the naloxone pretreatment group was 1. 24 times than that of the control group (all P < 0. 05 ) . (3) Naloxone inhibited hypoxia-induced NF-κB activation(P <0. 05). (4) The antioxidant, NAC, inhibited hypoxia-induced NF-κB activation with inhibition rate of 49% (P <0. 05). Conclusion Naloxone can prevent hypoxia-induced rat neuronal apoptosis by inhibiting hypoxia-induced ROS generation in rat neuron and further blocking NF-κB activation.
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