阿尔茨海默病患者血浆3-NT浓度的变化及其临床意义
The significance of determination of plasm 3-NT levels in patients with Alzheimer disease
殷晓菁 1胡玲玲 1彭誉 2周华 2邱晨红 2孙沁怡 2王锋 2谢红娜 2赵中2
作者信息
- 1. 无锡市第三人民医院神经内科,江苏无锡214041
- 2. 南京医科大学附属苏州医院神经内科,江苏苏州215001
- 折叠
摘要
目的 探讨血浆3-硝基酪氨酸(3-NT)浓度与阿尔茨海默病(AD)患者认知功能损害的关系.方法 应用简易精神状态检查量表(MMSE)检测入组患者的认知功能,将其分为3组,即正常对照组(NC,37例)、血管性痴呆组(VD组,41例)、AD组(48例),分别用酶联免疫吸附试验(ELSIA)测定3组患者的血浆3-NT浓度,并对AD组患者的血浆3-NT浓度与MMSE得分及各个认知领域分值进行相关性分析.结果 NC组、VD组、AD组患者血浆3-NT浓度分别为(55.09±9.63) nmol/1、(70.42±11.45) nmol/1、(119.46±21.82) nmol/1,AD组患者血浆3-NT浓度明显高于NC组、VD组(均P＜0.05)；AD组患者血浆3-NT浓度与MMSE得分成明显负相关(r=-0.638,P＜0.05)；AD组患者血浆3-NT浓度与记忆力、定向力、注意力和计算能力均成明显负相关(均P＜0.05).结论 血浆3-NT浓度测定有可能作为判断AD患者认知功能损害的生化指标,亦可作为AD与VD鉴别诊断的生物学指标.
Abstract
Objective To explore the relationship between plasm 3-nitrotyrosine (3-NT) levels and Alzheimer disease( AD). Methods The subjects were 48 patients with AD and 78 controls ,including 41 patients with vascular dementia (VD) and the 37 normal controls ( NC ). Cognitive function and plasm 3-NT levels in three groups were tested by Mini mental state examination ( MMSE) and enzyme linked immunosorbent assay(ELJSA) Respectively. Results The levels of plasm 3-NT were ( 119.46 ±21. 82) nmol/L in AD patients, (70. 42 ± 11. 45) nmol/L in VD, and (55. 09 ± 9. 63) nmol/L in NC, respectively (all P < 0.05). The correlation analysis revealed that the plasm 3-NT levels were negatively correlated with the total MMSE scores (r= -0. 638, P< 0.05). Multivariable linear regression showed that the plasm 3-NT levels were also negatively correlated with the memory scores,the orientation scores , the attention scores and the calculation in the AD groups(all P <0.05). Conclusion 3-NT may be used as the biochemical indicator for observing AD,and may be used for differentiating AD and VD.
关键词
阿尔茨海默病/血管性痴呆/3-硝基酪氨酸/简易精神状态检查量表Key words
Alzheimer disease/vascular dementia/3-nitrotyrosine/Mini mental state examination引用本文复制引用
基金项目
南京医科大学科技发展基金面上资助项目(2010NJMU107)
苏州市科技发展计划(社会发展、农业科技)项目(SYSD2011110)
出版年
2012
NETL
NSTL
维普
万方数据