Key targets of adenomyosis and screening of traditional Chinese medicines based on bioinformatics methods
Objective:By combining data mining with bioinformatics technology,the gene chip databases of adenomyosis patients and normal people were compared,the differential genes were searched,then the biological processes and potential targets involved in the differential genes were analyzed to predict the potential drugs.Methods:The GEO database was used to screen out the two data sets of GSE78851 and GSE7307,GEO2R was used to analyze the common differential genes of the chip,the differential gene heat map was generated by Omicshare.The GO and KEGG analysis of differential genes was performed by DAVID database,and the protein-protein interaction network was constructed by using String.The core gene was further subjected to modular analysis of differentially expressed genes using the MCODE plugin of Cytoscape 3.9.0.The drug for the treatment of adenomyosis was screened by the medical ontology information retrieval platform.Results:A total of 433 consensus differential genes were screened.The differentially expressed genes were mainly enriched in cell adhesion,estradiol response,apoptosis and other biological processes and oxidative phosphorylation,estrogen signaling pathway,PI3K-Akt and other signaling pathways.A total of 16 core genes were found,including P53 protein(TP53),cytochrome C(CYCS),actin(ACTB),type Ⅳcollagenase(MMP2),estrogen receptor(ESR1),CD44,mitogen-activated protein kinase 3(MAPK3),insulin-like growth factor Ⅰ(IGF-1),cytoplasmic dynein 1 heavy chain 1(DYNC1H1),thrombospondin-1(THBS1),cytochrome oxidase 4 Ⅱ(COX4Ⅱ),NDUFAB1,human fibrillin-1(FBN1),ATP5A1,NDUFB7,and ATP5G1.The module analysis was carried out in Cytoscape 3.9.0 and two important modules were obtained,and it was found that the module 1 gene was mainly related to the oxidative phosphorylation signaling pathway,and the module 2 gene was mainly related to the Rap1 and Wnt signaling pathways.The traditional Chinese medicines such as Salvia miltiorrhiza,Angelica,Poria,Sanqi,Magnolia,Camellia,and Silkworm were screened.Conclusions:The biological processes and signal pathways obtained by differentially expressed genes and enrichment analysis can provide a reference for potential targets of adenomyosis treatment.The potential drugs for adenomyosis are obtained through core gene mapping,which would provide new ideas for the treatment of adenomyosis.
万方数据
维普
