Weakened ovarian recovery function and increased oxidative stress level due to prenatal stress
Objective:A rat model of postpartum depression(PPD)induced by restraint stress during pregnancy was used to explore the effects of prenatal stress on ovarian reproductive hormone secretion and oxidative stress response.Methods:Rats were divided into two groups(6 rats per group).Those in the control group received no intervention and those subjected to restraint stress every 2 hours from 8 to 21 days of gestation were as the PPD group.The depressive behavior was detected within one week after delivery.The plasma levels of FSH and LH were detected by ELISA.The expression of ERα,ERβ,Nrf2,HO-1,ERK1/2,p-ERK1/2,IL-6 were detected by Western blot.HE staining was used to observe the development of follicles.Results:Compared with the control group,the preference rate of sugar and water in PPD group was significantly decreased(P＜0.01),and the immobility time of forced swimming was significantly prolonged(P＜0.001).Plasma FSH level decreased(P＜0.001),while LH level increased(P＜0.01).The protein levels of ERβ,p-ERK1/2,Nrf2 and HO-1 in ovary were decreased,but the protein level of IL-6 was increased(P＜0.05).HE staining showed that the number of atretic follicle of rat ovary in PPD group was increased.Conclusions:Restraint stress during pregnancy can increase oxidative stress response and inflammatory factors in ovary,suggesting that restraint stress during pregnancy can reduce the recovery ability of the ovary after delivery.
国家科技期刊平台
NSTL
万方数据
维普
