Effects of advanced maternal age on the proliferation and synaptic plasticity of neural stem cells in the prefrontal cortex of offspring rats
Objective:To investigate the impact of advanced maternal age on neural stem cell proliferation and synaptic plasticity in the prefrontal cortex of offspring rats.Methods:Female SD rats aged 3 months and 12 months were mated with male SD rats aged 3 months.The resulting offspring were divided into two groups:control group and advanced maternal age(AMA)group.Prefrontal cortical brain tissue was collected from newborns one day after birth,and HE staining was conducted to observe morphological changes in neurons of prefrontal cortex.qRT-PCR and Western blot techniques were utilized to measure mRNA and protein expression levels of G protein signaling regulator 2(RGS2),Nestin,postsynaptic density protein 95(PSD95),brain-derived neurotrophic factor(BDNF),and synaptophysin(SYN)in the prefrontal cortex.Results:Compared to the control group,HE staining revealed a reduction in the number of neurons in the prefrontal cortex of the AMA group,accompanied by neuronal degeneration characterized by cellular body swelling and vacuolization.qRT-PCR and Western blot analysis demonstrated significantly lower mRNA and protein expression levels of Nestin,RGS2,PSD95,BDNF,and SYN in the AMA group compared to the control group(P＜0.05).Conclusions:The proliferation and synaptic plasticity of neural stem cells in the prefrontal cortex are diminished in offspring from rats with advanced maternal age,potentially contributing to the developmental disorders of brain and cognitive decline.
国家科技期刊平台
NSTL
万方数据
维普
