Multivariate analysis of factors for outcomes of in vitro maturation culture of human immature oocytes
Objective:To explore factors for outcomes of in vitro maturation(IVM)culture of human immature oocytes(oocyte mature and day-3 available embryos)with multivariate factors analysis.Methods:The clinical data of patients with immature oocytes who underwent IVF/ICSI treatment at the Assisted Reproductive Center of Zhongshan City People's Hospital from June 2020 to June 2023 were collected.In accordance with the inclusion and exclusion criteria,a total of 207 immature oocytes from 45 patients were included in this study.IVM culture was conducted using both commercial IVM culture medium and self-made IVM culture medium.After 24 and 48 hours of culture,the status of the oocytes was assessed.Once oocytes maturing,ICSI,embryo culture and observation of the embryos were performed.The quantity of day-3 available embryos was documented.Then,logistic regression analysis was used to evaluate the influencing factors for the rates of oocyte maturation and day-3 available embryos,such as culture media,female age,infertility factors and body mass index(BMI)and so on.Results:A total of 207 immature oocytes were cultured for IVM in this study,resulting in 159 mature oocytes,with the mature rate of 76.81％,and 73 day-3 available embryos,with the day-3 available embryo formation rate of 35.27％.There was no significant difference in oocyte maturation rate among various IVM culture media,female age,ovulation induction protocols,BMI,basal FSH levels,basal LH levels,FSH/LH ratio,anti-Müllerian hormone(AMH)levels,years of infertility,types of infertility,factors for infertility,and status of immature oocytes(P＞0.05).The rate of day-3 available embryos using commercial IVM culture medium was 42.86％,which was significantly higher when compared to self-made IVM culture medium(27.45％,P＜0.05).There was no significant difference in the rate of day-3 available embryos among various female age,ovulation induction protocols,BMI and so on(P＞0.05).A multivariate logistic regression analysis revealed that factors,such as IVM culture medium,female age and so on,were not independent factors for oocyte maturation.Nonetheless,IVM culture medium and female age were found to be independent factors influencing the rate of day-3 available embryos(P＜0.05).Conclusions:When immature oocytes are triggered with HCG and undergo IVM,the use of commercial IVM medium may be more favorable for the formation of day-3 available embryos compared to self-made medium.Moreover,as the female age increases,the rate of day-3 available embryos derived from IVM mature oocytes,shows a decrease.
万方数据
维普
