The impact of ureaplasma urealyticum infection of reproductive tract and age on sperm DNA fragmentation index and routine semen parameters
Objective:To explore the effects of ureaplasma urealyticum(Uu)infection of reproductive tract and age on sperm DNA fragmentation index(DFI)and routine semen parameters.Methods:A total of 7 106 men who underwent premarital medical examination and preconception care examination on Physical Examination Department and Reproductive Health Department of Shenzhen Baoan Women's and Children's Hospital from January 2019 to July 2023 were recruited.Human semen was collected to analyze conventional sperm parameters and also Uu infection and sperm DFI with the cultivation method and flow cytometry.All the cases were classified into three groups based on their age for less than 30y,30-35y and over 35y.The discrepancy with various sperm parameters was evaluated.Results:Uu-positive rate was 11.89％among 5 years with uptrend overall while dropping at 2020.There were no significant differences in a positive rate of Uu among different seasons and ages.As for Uu-positive men,compared with those aged less than 30y and 30-35y,men aged over 35y had significantly lower sperm survival rate,percentage of forward sperm motility and total sperm count(P＜0.05)as well as significantly higher sperm DFI(P＜0.05).The age was positively correlated with sperm DFI among Uu-positive men(r=0.26,P=0.000 2).Logistic regression results showed that age[OR=1.393,95％CI(1.002,1.935),P=0.040],sperm survival rate[OR=0.746,95％CI(0.608,0.916),P=0.005]and total sperm count[OR=0.984,95％CI(0.968,0.999),P=0.040]were independently risk factors for sperm DFI among Uu-positive men(P＜0.05).Conclusions:Uu positive,along with advanced ages,notably resulted in abnormal routine semen characteristics and higher sperm DFI.Uu testing could be considered as a candidate screening indicator for semen quality as well as laboratory evidence for men's fertility evaluation.
万方数据
维普
