Lymphocyte subsets in peripheral blood and cytokine levels in autoantibody-positive patients with recurrent spontaneous abortion
Objective:To investigate the changes and significance of lymphocyte subsets and cytokine levels in the peripheral blood of autoantibody-positive patients with recurrent spontaneous abortion(RSA).Methods:The clinical data of 139 patients with RSA were retrospectively analyzed.According to the presence or absence of autoantibodies and the type of autoantibodies,the patients were divided into six groups as antibody negative control group(n=41),anti-thyroid antibody(ATA)positive group(n=22),anti-phospholipid antibodies(APLs)positive group(n=32),antinuclear antibody(ANA)positive group(n=21),ATA positive combined with APLs positive group(n=10),and ATA positive combined with ANA positive group(n=13).The proportion of lymphocyte subsets in peripheral blood and the levels of cytokines in serum were compared before pregnancy among all groups,and stratified analysis was made for each positive group according to different clinical characteristics..Results:The serum levels of interleukin(IL)-2 and IL-6 in ATA positive combined with ANA positive group were significantly higher than those in control group and ATA positive group(P＜0.05).The level of tumor necrosis factor(TNF)-α in the serum of ATA positive combined with APLs positive group was higher than those in antibody negative control group,ANA positive group,and APLs positive group(P＜0.05).In subgroup analysis,the CD4+/CD8+ratio of high thyroid stimulating hormone(TSH)subgroup in ATA positive group was higher than that of normal TSH subgroup(P＜0.05),and the level of IL-4 was significantly lower than that of normal TSH subgroup(P＜0.05).The CD4+/CD8+ratio in the non-criteria APLs positive subgroup was lower than that in the criteria APLs positive subgroup(P＜0.05),and the proportion of CD8+T cells showed an increasing trend(P＞0.05).The CD4+/CD8+ratio in the ANA positive group with a titer of 1∶320 was significantly lower than that in the ANA positive group with a titer of 1∶100(P＜0.05).Conclusions:The immune function of RSA patients with different types of autoantibodies is disturbed,which is mainly related to abnormal cytokine levels and the imbalance of CD4+/CD8+ratio.
万方数据
维普
