The effect of partial deletions in the azoospermia factor C subregion of the Y chromosome on reproductive outcomes of ICSI among azoospermia and severe oligozoospermia patients
Objective:To investigate the effect of partial deletions in the azoospermia factor c subregion(AZFc)of the Y chromosome on reproductive outcomes of ICSI among azoospermia and severe oligozoospermia patients.Methods:Clinical data of men suffering from azoospermia and severe oligozoospermia at Reproductive Center of the 900th Hospital from January 2012 to October 2023 were retrospectively analyzed.The patients were divided into a deletion group(partial deletion of AZFc region,n=34)and a control group(no deletion seen,n=46)according to the presence or absence of deletion of the AZFc region.The deletion group was then categorized into gr/gr deletion subgroups(n=22)and b2/b3 deletion subgroups(n=12)based on the deletion type.Embryonic development and clinical pregnancy outcomes were compared and analyzed among the groups.Results:The normal fertilization rate in the deletion group was significantly lower than that in the control group(60.61％vs.74.44％,P＜0.01),but there were no significant differences in bipronuclear(2PN)cleavage rate,high-quality embryo rate,positive rate of HCG and clinical pregnancy rate(P＞0.05).Subgroups in the deletion group had significantly lower normal fertilization rates in both the gr/gr deletion subgroup and the b2/b3 deletion subgroup compared with the control group[63.75％,54.47％vs.74.44％,P＜0.05],while the differences in 2PN cleavage rate and the rate of high-quality embryos were not statistically significant(P＞0.05).And there were no significant differences in fertilization and embryo development between the two deletion subgroups(P＞0.05).Conclusions:During ICSI for azoospermia and severe oligozoospermia,AZFc gr/gr deletion and AZFc b2/b3 deletion could affect normal fertilization rate but not the clinical outcomes of ICSI.
Azoospermia factor C subregionPartial deletionARTICSI
万方数据
维普
