Effect of berberine on intrauterine adhesion and vaginal microbiota of rats
Objective:To investigate the effect of berberine(BBR)on intrauterine adhesion(IUA)and vaginal microbiota of rats.Methods:Twenty SPF-grade healthy female SD rats with 6-week-old were randomly divided into sham group(n=5),model group(n=5),estradiol valerate(EV)group(n=5)and BBR group(n=5).IUA models of the model group,EV group and BBR group were established by mechanical damage combined with infection.EV of 0.63 mg·kg-1·d-1was given intragastric administration to EV group and BBR of 100 mg·kg-1·d-1to BBR group once a day for 28 days.The serum,vaginal washing fluid and uterine tissue samples were collected.The general state,uterine morphology and pathology were observed.The uterine index,uterus patency and American Fertility Society(AFS)scores,the number of endometrial glands,endometrial thickness,the fibrosis rate,expression levels of serum inflammatory factors and vaginal microbiota situation were evaluated.Results:Comparing with the sham group,the uterine index,the fibrosis rate,uterus patency and AFS scores,expression levels of serum tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-6,the content of vaginal staphylococcus aureus and colibacillus as well as Simpson index of the model group were all significantly increased(P＜0.05),while the number of endometrial glands,endometrial thickness,lactobacillus and bifidobacteria levels as well as bacteria abundance and uniformity,Shannon-Wiener index were all decreased(P＜0.05).Comparing with the model group,the uterine index,the fibrosis rate,uterus patency and AFS scores,expression levels of serum TNF-α,IL-1β and IL-6,the content of vaginal staphylococcus aureus and colibacillus as well as Simpson index of BBR group were all significantly decreased(P＜0.05),while the number of endometrial glands,endometrial thickness,the content of lactobacillus and bifidobacteria,bacteria abundance and uniformity,Shannon-Wiener index were all increased(P＜0.05).Conclusions:BBR has superior curative effect on IUA model rats,and the IUA state is obviously improved,which potential mechanism might be related with the amelioration of vaginal microbiota disturbance by BBR.
万方数据
维普
