Objective:To compare the temperature changes of liquid droplets in different types of culture dishes on heating stages with different temperature.Methods:Three different brands(Vitrolife group,Oosafe group,Falcon group)of growth dishes,center-well dishes,four-well dishes and ICSI dishes were selected as the study objects.The different types of culture dishes were prepared to replicate the procedures performed by embryologists.The temperature changes of culture droplets were recorded within 5 minutes on the heating stage at 37℃ and 38.5℃ respectively.Results:On the heating stage at 37℃,the temperature of the culture medium in various types of culture dishes was gradually decreasing with time.Compared with the Vitrolife group,the culture medium temperature decreased more rapidly in growing dish and four-well dish without cover oil in Falcon group(P＜0.05).The temperature of the culture medium in the centre-well dish decreased faster in the Oosafe group than that in the Vitrolife group(P＜0.05).The culture droplet temperature of ICSI dishes in Falcon and Oosafe groups dropped faster than that in Vitrolife group(P＜0.05).However,there was no significant difference in the culture droplet temperature of three groups of four-well plates after oil(P＞0.05).Additionally,on the heating stage at 38.5℃,the temperature of the culture medium in the four-well dish without the oil and the central well dish continued to decrease,while the temperature in the oil-covered growth dish,four-well dish and ICSI dish increased.There was no significant difference in the culture droplet temperature of growth dish between the Falcon group and Vitrolife group(P＞0.05),while the temperature of growth dish in the Oosafe group increased relatively slowly(P＜0.05).Furthermore,both Falcon and Oosafe group exhibited a more rapid loss of the medium temperature of four-well dish without the oil and centre-well dish compared to Vitrolife group(P＜0.05).The temperature of the culture droplet in the ICSI dish in the Vitrolife group increased faster than that in the other two groups(P＜0.05).Conclusions:The setting temperature of the heating stage needed to reach the optimum temperature of the culture droplet in different types of culture dishes may be different.In practical applications,the temperature setting of the workstation heating stage should consider both actual usage and various types of culture dishes employed,ultimately being determined based on the embryonic development and clinical outcomes for patients.
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