首页|iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet

iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie diet-induced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses.In the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)groups.Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d.The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d.Pathological evaluation of the lung tissue was performed.Differentially expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics analyses.The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)Tools.DEPs were verified by western blot.Results:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue.The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M groups.Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative phosphorylation.The protein-protein interaction network revealed that Atp5a1.Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction.Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to Atp5a1.Conclusion:The Yinlai decoction alleviated lung tissue damage and pneumonia in mice that were fed a high-calorie diet by regulating the TCA and oxidative phosphorylation.Our study highlights the importance of a healthy diet for patients with pneumonia and provides a scientific basis for the pre-vention and treatment of pneumonia through dietary adjustments.

Yinlai decoctionHigh-calorie dietPneumoniaiTRAQ proteomicsEnergy metabolism

Qianqian Li、Tiegang Liu、Chen Bai、Xueyan Ma、Hui Liu、Zi'an Zheng、Yuxiang Wan、He Yu、Yuling Ma、Xiaohong Gu

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School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China

Institute of Chinese Medicine Epidemic Disease,Beijing University of Chinese Medicine,Beijing 102488,China

School of Traditional Chinese Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

Department of Acupuncture and Mini-invasive Oncology,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China

OCMRC Center,University of Oxford,Oxford OX1 3PT,United Kingdom

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国家自然科学基金Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine

81874421ZYYCXTD-C-202006

2024

10.1016/j.jtcms.2023.12.003

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(1)
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