首页|GPCR-Gs mediates the protective effects of ginsenoside Rb1 against oxygen-glucose deprivation/re-oxygenation-induced astrocyte injury

GPCR-Gs mediates the protective effects of ginsenoside Rb1 against oxygen-glucose deprivation/re-oxygenation-induced astrocyte injury

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  • 国家科技期刊平台
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Objectives:To investigate whether the protective actions of ginsenoside Rb1(Rb1)on astrocytes are mediated through the Gs-type G-protein-coupled receptor(GPCR-Gs).Methods:Primary astrocyte cultures derived from neonatal mouse brain were used.Astrocyte injury was induced via oxygen-glucose deprivation/re-oxygenation(OGD/R).Cell morphology,viability,lactate de-hydrogenase(LDH)leakage,apoptosis,glutamate uptake,and brain-derived neurotrophic factor(BDNF)secretion were assessed to gauge cell survival and functionality.Western blot was used to investigate the cyclic adenosine monophosphate(cAMP)and protein kinase B(Akt)signaling pathways.GPCR-Gs-spe-cific inhibitors and molecular docking were used to identify target receptors.Results:Rb1 at concentrations ranging from 0.8 to 5 μM did not significantly affect the viability,glutamate uptake,or BDNF secretion in normal astrocytes.OGD/R reduced astrocyte viability,increasing their LDH leakage and apoptosis rate.It also decreased glutamate uptake and BDNF secretion by these cells.Rb1 had protective effects of astrocytes challenged by OGD/R,by improving viability,reducing apoptosis,and enhancing glutamate uptake and BDNF secretion.Additionally,Rb1 activated the cAMP and Akt pathways in these cells.When the GPCR-Gs inhibitor NF449 was introduced,the protective effects of Rb1 completely disappeared,and its activation of cAMP and Akt signaling pathways was significantly inhibited.Conclusion:Rb1 protects against astrocytes from OGD/R-induced injury through GPCR-Gs mediation.

GinsengGinsenoside Rb1ReceptorGPCRAstrocytesNeuroprotective effects

Xi Wang、Ying Liu、Juan Li、Jiayu Xie、Yi Dai、Minke Tang

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School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China

Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China

School of Medicine,Hyogo Medical University,Nishinomiya 663-8501,Japan

grant International Cooperation Project of Prevention and Treatment of Major Diseases with Chinese MedicineHighend Experts Support Program from the Ministry of Science and TechnologyBasic Research Funds from the Ministry of Education

GZYYGJ2021047DL 2021110001L1000061223731

2024

10.1016/j.jtcms.2023.11.002

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(1)
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