首页|Mechanism of action of Wuzi Yanzong pill in the treatment of oligoasthenozoospermia in rats determined via serum metabolomics

Mechanism of action of Wuzi Yanzong pill in the treatment of oligoasthenozoospermia in rats determined via serum metabolomics

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  • 国家科技期刊平台
  • NETL
  • NSTL
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Objective:To investigate the mechanism of action of Wuzi Yanzong pill(WYP)in rats with oligoasthe-nozoospermia(OAZ)via metabolomics and to provide a possible basis for improving this WYP-based treatment.Methods:A rat model of OAZ was established by treating male Sprague-Dawley rats with glucosides from Tripterygium wilfordii Hook.F.Seventy-two rats were randomly divided into six groups:control,L-carnitine(positive control),model,and low-,medium-,and high-dose WYP groups.Rats in the experimental groups were treated with WYP for 4 weeks.At the end of the treatment period,sperm cell quality(density,motility,and viability)was assessed using a semen analysis system,mitochondrial membrane potential(MMP)was assessed using flow cytometry,and testicular injury was assessed using hematoxylin and eosin staining to validate the therapeutic effect of WYP in OAZ.Further,serum metabolomics-based analysis was performed using high-performance liquid chromatography-mass spectrometry to identify differential metabolic pathways and possible mechanisms of action of WYP in OAZ treatment.Results:A rat model of OAZ was considered successfully-established after comparing the quality of spermatozoa in the model group to that in the control group.WYP-M and WYP-H treatments signifi-cantly improved sperm cell density,motility,and viability compared with those in the model group(all P＜.05).Compared with the model group,both WYP-M and WYP-H treatments increased MMP values(P=.006 and P=.021 respectively),while there was no significant difference in the L-carnitine group.L-carnitine and WYP administration reversed damage to the testes to varying degrees compared with that in the model group.Further,44 differential metabolites and four metabolic pathways,especially autophagy pathway,related to OAZ were identified via metabolomics.Conclusions:WYP improves sperm cell quality and MMP in OAZ primarily via autophagy regulation.These findings can be employed to improve the efficacy of WYP in humans.

Wuzi Yanzong pillOligoasthenozoospermiaSperm cell qualityMetabolomicsAutophagyMale infertility

Zhenru Shen、Zhaohua Zhang、Kejin Tong、Chunrui Wang、Shuaiqiang Wang、Ping Zhao、Meng Gu、Jingjing Hu、Yibo Tang、Zhenquan Liu

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School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,102488,China

Beijing Tong Ren Tang Company Limited,Beijing,100062,China

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China

China Beijing Tong Ren Tang Group Co.,Ltd.,Beijing,100075,China

Faculty of Chinese Medicine,Macau University of Science and Technology,Macau,999078,China

Department of Pathology,University of California San Diego,La Jolla,CA,92093,USA

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Longitudinal Development Project of the Beijing University of Chinese MedicineLongitudinal Development Project of the Beijing University of Chinese MedicineLongitudinal Development Project of the Beijing University of Chinese Medicine

2018-zxfzjj-002BeijingChina

2024

10.1016/j.jtcms.2024.03.002

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(2)
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