首页|Mechanism of acupoint penetration acupuncture therapy regulating chondrocyte autophagy via the PI3K/Akt-mTOR pathway in KOA rats

Mechanism of acupoint penetration acupuncture therapy regulating chondrocyte autophagy via the PI3K/Akt-mTOR pathway in KOA rats

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Objective:To investigate whether acupoint penetration acupuncture(APA)could regulate chondrocyte autophagy and apoptosis via the PI3K/Akt-mTOR signaling pathway to reduce cartilage degeneration in knee osteoarthritis(KOA)rats.Methods:KOA was induced in rats via intra-articular injection of sodium iodoacetate resolution.Forty male Sprague-Dawley rats were randomly assigned to blank control,model,APA,electro-acupuncture(EA),and sham model groups(n=8)and those in the APA and EA groups received their respective therapies.Following completion of the treatment course,histological examinations of cartilage and muscle were conducted.Levels of apoptosis-and autophagy-related factors,including Bax,Bcl-2,mTOR,ULK-1,and Beclin-1 protein,and mRNAs were assessed.Additionally,β-endorphin(β-EP)concentrations in the brain and serum were measured.Results:Histological analysis revealed that APA alleviated cartilage and muscle damage compared with the model group.APA inhibited cartilage degeneration by modulating the expression of apoptosis-and autophagy-related proteins and mRNA,thus preventing chondrocyte apoptosis.In the APA group,Bax and mTOR protein levels were significantly lower than those in the model group(both P=.024).Conversely,the Bcl-2 expression level was significantly higher than that in the EA group(P=.035).Additionally,ULK-1 expression was significantly lower than that in the EA group(P=.045).The mRNA level of Bax was significantly higher than that in the blank control group(P＜.001).However,Beclin-1 levels were significantly higher than those in both the model and EA groups(both P＜.001).ELISA results showed a significant decrease in the concentration of β-EP in the brains of the rats in the APA group compared with those in the model group(P=.032).Conclusions:APA reduced osteoarthritis-related pain and alleviated cartilage damage by upregulating chondrocyte autophagy and down-regulating apoptosis via signaling pathways involving PI3K/Akt-mTOR in KOA rats.

Acupoint penetration acupunctureKnee osteoarthritisChondrocyteAutophagyApoptosisCell proliferation

Yang Gao、Qingbo Wang、Songwei Li、Xiaojing Shi、Shan Dai、Jingjing Yu、Qingpan Zhao、Yang Wang、Youlong Zhou

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Department of Acupuncture and Moxibustion Section,The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China

Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou 450053,China

Academy of Medical Sciences,Zhengzhou University,Zhengzhou 450052,China

Department of Anesthesiology,The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China

The First Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450018,China

School of International Education,Henan University of Chinese Medicine,Zhengzhou 450018,China

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2024

10.1016/j.jtcms.2024.04.002

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(3)