首页|Acupotomy ameliorates knee osteoarthritis-related collagen deposition and fibrosis in rabbit skeletal muscle through the TGF-β/Smad pathway

Acupotomy ameliorates knee osteoarthritis-related collagen deposition and fibrosis in rabbit skeletal muscle through the TGF-β/Smad pathway

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Objective:To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis(KOA)model.Methods:Rabbits(n=18)were randomly divided into control,KOA,and KOA+acupotomy(Apo)groups(n=6).The rabbits in the KOA and Apo groups were modeled using the modified Videman's method for 6 weeks.After modeling,the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis,vastus lateralis,rectus femoris,biceps femoris,and anserine bursa tendons around the knee.The behavior of all animals was recorded,rectus femoris tissue was obtained,and histomorpho-logical changes were observed using Masson staining and transmission electron microscopy.The expression of transforming growth factor-β1(TGF-β1),Smad 3,Smad 7,fibrillar collagen types Ⅰ(Col-Ⅰ)and Ⅲ(Col-Ⅲ)was detected using Western blot and real-time polymerase chain reaction(RT-PCR).Results:Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris,compared with the KOA group(P=.034).Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA,thus preventing skeletal muscle fibrosis.Western blot and RT-PCR analysis revealed that in the Apo group,Col-Ⅰ,and Col-Ⅲ protein levels were significantly lower than those in the KOA group(both P＜.01),same as Col-Ⅰ and Col-Ⅲ mRNA levels(P=.0031;P=.0046).Compared with the KOA group,the protein levels of TGF-β1 and Smad 3 were significantly reduced(both P＜.01),as were the mRNA levels of TGF-β1 and Smad 3(P=.0007;P=.0011).Conversely,the levels of protein and mRNA of Smad 7 were significantly higher than that in the KOA group(P＜.01;P=.0271).Conclusion:Acupotomy could alleviate skeletal muscle fibrosis and delay KOA progress by inhibiting collagen deposition through the TGF-β/Smad pathway in the skeletal muscle of KOA rabbits.

AcupotomyKnee osteoarthritisSkeletal muscleFibrosisCollagen deposition

Tingyao Hu、Einar Khavaza、Chuxi Liang、Longfei Xing、Xilin Chen、Yue Xu、Weiwei Ma、Farid Mokhtari、Juan Lu、Changqing Guo

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School of Acupuncture,Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,102488,China

Department of Traditional Chinese Medicine,Peking University People's Hospital,Beijing 100044,China

The Fifth College of Clinical Medicine,Guangzhou University of Traditional Chinese Medicine,Guangzhou 511436,China

Department of Acupuncture and Rehabilitation,Guangdong Second Hospital of Traditional Chinese Medicine,Guangzhou 510260,China

Traditional Clinic,Shahid Beheshti Medical University,Tehran 1698633631,Iran

Department of Traditional Chinese Medicine,Shenzhen Hospital of Southern Medical University,Shenzhen 510515,China

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2024

10.1016/j.jtcms.2024.06.001

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(3)