首页|Effects of acupuncture on serotonin,histamine,substance P,and tryptase levels at sensitized points in model rats with knee osteoarthritis

Effects of acupuncture on serotonin,histamine,substance P,and tryptase levels at sensitized points in model rats with knee osteoarthritis

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  • 国家科技期刊平台
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  • NSTL
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Objective:To elucidate the differences in manual acupuncture effectiveness at sensitized points by investigating the mechanisms of local skin action at different sensitization points in rats with knee osteoarthritis(KOA).Methods:Forty Sprague-Dawley rats were equally divided into control,model(1 mg of mono-iodoacetate into the right knee joint cavity),sham operation,manual acupuncture at right Tianjing acupoint(MAR-SJ 10),and left SJ 10 groups.Safranine-O and fast green staining were used to assess the modeling.The morphological and functional changes in mast cells(MCs)were assessed during acupoint sensitization using toluidine blue and immunofluorescence staining.The levels of serotonin,histamine,substance P(SP),and tryptase at skin acupoints and serum levels of IL-β,IL-6,and TNF-α were detected using ELISA.Results:After 14 days of treatment,the number of MCs and their degranulation rates were statistically higher in the model group than in the control group(both P＜.001).After applying acupuncture,the levels of 5-HT,HA,and SP at skin acupoints were lower than those in the model group(all P＜.05),and tryptase level was higher(both P＜.05).Tryptase level was higher on the skin at the MAL-SJ10 acupoint than that on the MAR-SJ 10 acupoint(P=.004).Compared with the model group,the serum levels of IL-1β,IL-6,and TNF-α in the MAR-SJ 10 and MAL-SJ 10 groups were lower(all P＜.05).Conclusion:Acupuncture at KOA-sensitized acupoints mitigates joint injury in KOA rats and may bidi-rectionally regulate local MCs of these acupoints.This finding not only enhances the reference value of sensitizing points in clinical diagnosis and treatment,but also contributes to the understanding of the biological mechanisms underlying acupuncture intervention at sensitizing points.

Knee osteoarthritisAcupunctureAcupoint sensitizationMast cellsSerotoninHistamineSubstance PTryptase

Jiayi Yang、Zidong Wang、Jing Jiang、Huiling Tian、Shun Wang、Yizhi Liu、Zumao Cao、Changqing Joseph Yang、Zhigang Li

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School of Acupuncture,Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,102488,China

School of Nursing,Beijing University of Chinese Medicine,Beijing 102488,China

School of Traditional Chinese Medicine,Capital Medical University,Beijing,100050,China

Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China

Dongzhimen Hospital Key Laboratory,Beijing University of Chinese Medicine,Beijing 100700,China

American Institute of Mental Health in Traditional Chinese Medicine,Los Angeles,CA 90025,USA

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2024

10.1016/j.jtcms.2024.09.004

中医科学杂志(英文)
北京中医药大学,清华大学出版社有限公司

中医科学杂志(英文)

ISSN：2095-7548
年,卷(期)：2024.11(4)