首页|The role of brevican regulation in the antidepressant effects of electroacupuncture in a chronic stress rat model
The role of brevican regulation in the antidepressant effects of electroacupuncture in a chronic stress rat model
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国家科技期刊平台
NETL
NSTL
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Objective:To investigate the mechanism of electroacupuncture(EA)for treating depression and to explore the role of brevican in the medial prefrontal cortex(mPFC)in modulating stress susceptibility and the antidepressant effects of EA in rats.Methods:Twenty-four Sprague-Dawley(SD)rats were equally divided into three groups:green fluo-rescent protein(GFP)+control,GFP+chronic unpredicted mild stress(CUMS),and short-hairpin RNA targeting on brevican(shBcan)+CUMS.Another 24 SD rats were equally divided into CUMS+GFP,CUMS+GFP+EA,and CUMS+shBcan+EA groups.Behavioral tests were conducted to assess depression-like behavior.Western blot analysis was used to evaluate the expression of brevican,aggrecan,GLuA1,and PSD95 in mPFC subregions.Results:Behavioral parameter evaluation show that rats in the shBcan+CUMS group exhibited a significantly reduced sucrose preference(P=.0002)and increased immobility time(P=.0011)compared to those in rats in the GFP+CUMS group.Western blotting showed that brevican expression was significantly downregulated in the PrL of the shBcan+CUMS group compared with that in the GFP+CUMS group(P=.0192).Furthermore,compared to the CUMS+GFP+EA group,the CUMS+shBcan+EA group exhibited a significantly decreased sucrose preference(P=.0334),increased immobility time(P=.0465),and increased latency to food(P=.0261).In the CUMS+shBcan+EA group,the EA-induced brevican and PSD95 overexpression was reversed,compared with that in the CUMS+GFP+EA group(P=.0454 and P=.0198,respectively).Conclusion:EA exerts its antidepressant effects through the modulation of brevican expression in rats.Our findings highlight the important role for brevican in stress susceptibility,which could be a potential target for treating depression.
国家科技期刊平台
NSTL
万方数据
