首页|Mechanism of Jiawei Sanpian decoction in treating migraine through network pharmacology,molecular docking,and experimental studies
Mechanism of Jiawei Sanpian decoction in treating migraine through network pharmacology,molecular docking,and experimental studies
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Mechanism of Jiawei Sanpian decoction in treating migraine through network pharmacology,molecular docking,and experimental studies
Objective:To analyze and validate how Jiawei Sanpian decoction treats migraines by integrating network pharmacology,molecular docking technology,and experimental studies.Method:Using network pharmacology,the chemical components and core target proteins of the Jiawei Sanpian decoction were analyzed.Key chemical components were docked with core targets using mo-lecular docking,and the results were visualized.Nitroglycerin was injected into the dorsal cervical region to establish a rat migraine model.Finally,experiments were conducted to verify the effects of Jiawei Sanpian on related pathways and targets.Results:Four notable chemical components were identified,namely,β-sitosterol,quercetin,mairin,and kaempferol.Five representative targets were identified,namely,insulin-like growth factor 1(IGF-1),matrix metallopeptidase 2(MMP-2),interleukin-2(IL-2),superoxide dismutase 2(SOD2),and inducible nitric oxide synthase(NOS2).Molecular docking results revealed that the minimum binding energies between the four chemical components and the five targets were below-5 kcal/mol,indicating favor-able binding activity.Enzyme linked immunosorbent assay(ELISA)results demonstrated the efficacy of high-dose Jiawei Sanpian decoction in treating migraine by targeting IGF-1,IL-2,MMP-2,and SOD2(P＜.001).Real-time quantitative polymerase chain reaction(RT-qPCR)results demonstrated the effectiveness of high-dose Jiawei Sanpian decoction in treating migraine by targeting IGF-1,IL-2,MMP-2,and SOD2(P＜.001).After using erastin,the therapeutic effect of Jiawei Sanpian decoction declined.Conclusion:This study provides initial insights into the complex and multilayered therapeutic mecha-nisms of Jiawei Sanpian decoction in treating migraine,primarily through its diverse components,tar-gets,and pathways.These findings indicate that Jiawei Sanpian decoction may exert its effects mainly through processes linked to the mitochondrial inflammatory pathway,thereby providing therapeutic benefits for migraine.
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