Objective:To investigate the clinical significance of Interleukin 2(IL-2),IL-4,IL-6,IL-10,tumor necrosis factor-α(TNF-α)and interferon-γ(INF-γ)in peripheral blood of patients with thoracic malignant tumor before and after radiotherapy.Methods:According to the strict inclusion and exclusion criteria,50 patients with thoracic malignant tumor admitted to Lianshui People's Hospital from October 2021 to May 2023 were selected as the tumor group,and 30 healthy patients in the same period were selected as the control group.Peripheral venous blood was collected from 5 observation points in the tumor group before and during radiotherapy(3 weeks after radiotherapy),at the end of radiotherapy,1 month and 4 months after radiotherapy,and the contents of IL-2,IL-4,IL-6,IL-10,TNF-α and INF-γ in peripheral blood of patients before and after radiotherapy were detected by flow cytometry and compared with those of the controls group.Results:Compared with the control group,the levels of IL-2 and IFN-γ in peripheral blood of tumor group were significantly lower than those of the healthy group,and the levels of IL-6 and TNF-α were significantly higher than those of healthy group,with significant statistical differences(P＜0.05).The levels of IL-4 and IL-10 were not significantly different between the two groups(P＞0.05).During the period of radiotherapy,peripheral blood TNF-α in the tumor group began to increase after radiotherapy,and reached the highest level 1 month after radiotherapy,which was significantly higher than before radiotherapy(P＜0.05),and then gradually decreased,and no significant difference was found 4 months after radiotherapy compared with before radiotherapy(P＞0.05).In the tumor group,IL-6 in peripheral blood began to increase after radiotherapy,reached a peak at the end of radiotherapy and began to decrease,and there was no significant difference 4 months after radiotherapy compared with before radiotherapy(P＞0.05).There were significant differences in the levels of IL-6 and TNF-α in peripheral blood between the radiation induced lung injury(RILI)group and the non-RILI group at different periods of radiotherapy,and the levels of IL-6 and TNF-α increased in the RILI group at different periods of radiotherapy.There were no significant differences in the levels of IL-2,IL-4,IL-10 and IFN-γ between the two groups at different periods of radiotherapy.Conclusion:Radiotherapy will affect the levels of peripheral blood cytokines in patients with thoracic tumors to varying degrees.Monitoring the concentration of cytokines during radiotherapy can provide early warning for the immune crisis state of the body and lung radiation damage,and provide theoretical basis for the implementation of early immune prevention and corrective measures during radiotherapy.
malignant tumorradiotherapyhelper T lymphocyte 1 cytokinehelper T lymphocyte 2 cytokineradiation induced lung injury
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