Objective:To analyze the antibacterial activity of ceftazidime/avibactam(CZA)against carbapenem-resistant Klebsiella pneumoniae(CRKP)in vitro.Methods:Non-repetitive CRKP strains isolated from clinical departments in the First Affiliated Hospital of Kunming Medical University from January to December 2022 were collected,analyze the clinical distribution and sensitivity of CRKP;PCR was used to detect common carbapenemase genes in CRKP,CZA MIC against CRKP carrying different carbapenemase was determined by the micro broth dilution method,expressed as MIC50,MIC90 and MICrange;additionally,micro-checkerboard dilution method and time-kill assay were used to detect the antibacterial activity of CZA combined with aztreonam(ATM)on the NDM-producing CRKP.Results:A total of 143 non-repetitive CRKP strains were collected,the specimen was dominated by respiratory specimens,mainly concentrated in ICU;CRKP are characterized by extensive drug resistance,including resistance rate of Carbapenem was＞95％;143 CRKP carrying blaKPC and blaOXA-48 were 100％sensitive to CZA,blaNDM were 100％resistant to CZA,however,CZA combined with ATM showed a strong synergistic bactericidal effect on NDM-producing CRKP in vitro.Conclusion:It is necessary to detect the enzyme type of CRKP in the process of treatment with CZA,to provide drug guidance for clinical anti-infection treatment.
NETL
NSTL
万方数据
