Objective:To analyze the expression of RHOJ in patients with gastric cancer and its relationship with clinicopathological features,prognosis and immune infiltration of tumor microenvironment based on database,and to explore the related mechanism.Methods:By searching UALCAN and HPA databases,we investigated the differential expression of RHOJ in gastric cancer and normal tissues,and the subcellular expression of RHOJ.The TCGA database and GEO database were searched.RNA-Seq data and clinical baseline data of 415,76 and 434 cases of gastric cancer tissues were downloaded from Omnibus database GSE84426 and GSE84437,respectively.The relationship between RHOJ expression and clinicopathological features in TCGA and GEO databases was analyzed.The correlation between RHOJ expression in TCGA database and clinical baseline data and prognosis was analyzed by Cox regression analysis.KM-plotter database was used to analyze the relationship between RHOJ expression and prognosis of gastric cancer patients,and the survival curve and immune treatment-related receiver operating characteristic(ROC)curve were drawn.TISIDB database was searched to evaluate the relationship between RHOJ expression and immune invasion of gastric cancer microenvironment.Proteins associated with RHOJ were analyzed through STRING database.Gene ontology(GO)function annotation and KEGG pathway enrichment analyses were also conducted.Results:Compared with the normal control group,RHOJ had a low expression in gastric cancer tissues,which was related to immune invasion of gastric cancer tumors and was helpful to evaluate the efficacy of immunotherapy for gastric cancer.The low expression of RHOJ in patients with gastric cancer was an indicator of good prognosis.Conclusion:RHOJ can be used as a biological marker affecting the prognosis of patients with gastric cancer,and has a good diagnostic value.
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