Objective:To investigate the impacts of Echinacetin(ECH)on the proliferation,migration,and differentiation of dental pulp stem cells(DPSCs)by regulating the CXC chemokine ligand 12(CXCL12)/CXCR4 signaling pathway.Methods:DPSCs were studied and subjected to different concentrations of ECH(0.01 mg·mL-1,0.1 mg·mL-1,1 mg·mL-1),and they were grouped into low ECH concentration(ECH-L)group,medium ECH concentration(ECH-M)group,and high ECH concentration(ECH-H)group;DPSCs were treated with 1 mg·mL-1ECH and 100 nmol·L-1 AMD3100 simultaneously,and recorded as the ECH-H+AMD3100 group,and untreated DPSCs were used as blank control group;MTT method was applied to detect cell proliferation;Transwell experiment was applied to detect cell migration;Western blot was applied to detect the expression of CXCL12 and CXCR4 proteins;21 days after osteogenic induction,alizarin red S staining was applied to evaluate the formation of mineralized nodules;alkaline phosphatase(ALP)assay kit was applied to analyze ALP activity;qRT-PCR was applied to detect the expression levels of dentin matrix protein 1(DMP1),osteocalcin(OCN),and ALP mRNA.Results:Compared with the blank control group,the expression of CXCL12,CXCR4 protein,migration number,ALP content,mineralized nodule content,A450 value,expression levels of DMP1,OCN,and ALP mRNA in the ECH-L group,ECH-M group,and ECH-H group obviously increased,there were statistical differences among different concentrations of ECH(P＜0.05);compared with the ECH-H group,the expression of CXCL12,CXCR4 protein,migration number,ALP content,mineralized nodule content,A450value,expression levels of DMP1,OCN,and ALP mRNA in the ECH-H+AMD3100 group obviously decreased(P＜0.05).Conclusion:ECH promotes the proliferation,migration,and differentiation of DPSCs by up-regulating the CXCL12/CXCR4 signaling pathway.
维普
万方数据
