Objective:To investigate the effect of downregulating USP37 expression on programmed death-ligand 1(PD-L1)expression in triple-negative breast cancer(TNBC)cells and its role in tumor immune response.Methods:Immunohistochemistry was used to detect the expression of USP37 and its correlation with PD-L1 expression in TNBC tissues.siRNA-mediated transient transfection was performed to downregulate USP37 expression in MDA-MB-231 cells.qRT-PCR and Western Blot were used to measure the mRNA and protein levels of USP37 and PD-L1 in MDA-MB-231 cells after transient transfection.MDA-MB-231 cells transfected with siRNA were co-cultured with peripheral blood mononuclear cells(PBMCs),and CCK8 assay was used to evaluate the cytotoxic effect of PBMCs on MDA-MB-231 cells.Flow cytometry was used to detect changes in PD-L1 expression on MDA-MB-231 cells.Results:Immunohistochemistry showed that USP37 was highly expressed in TNBC tissues and positively correlated with PD-L1 expression(P<0.01).Compared to the control group,downregulation of USP37 significantly reduced the mRNA and protein levels of both USP37 and PD-L1 in MDA-MB-231 cells.CCK8 assay indicated that downregulation of USP37 enhanced PBMC-mediated cytotoxicity against MDA-MB-231 cells(P<0.01).Flow cytometry showed that the expression of PD-L1 on the surface of MDA-MB-231 cells was significantly reduced in the USP37 downregulation group(P<0.01).Conclusion:Downregulation of USP37 inhibits PD-L1 expression on MDA-MB-231 cells and influences the anti-tumor immune response in TNBC.
triple-negative breast cancerUSP37programmed death-ligand 1anti-tumor immune response
NETL
NSTL
万方数据
