枸杞提取物中玉米黄质和叶黄素含量的高效液相测定方法

Determination of Zeaxanthin and Lutein in Lycium Barbarum Extract by HPLC

唐瑗 ¹康瑶 ¹於洪建 ²张东星²

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作者信息

1. 天津中医药大学天津 301617
2. 天津益倍生物科技集团有限公司天津 300457
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摘要

选用色谱柱Innoval ODS-2(4.6 mm×250 mm,5 µm),以 0.05%三乙胺-乙腈为流动相,流速为 0.8 mL/min,柱温为28℃,在 450 nm波长处测定样品.结果显示:玉米黄质在 0.207～20.7 µg/mL范围内线性关系良好,判定系数r2=1.000 0,平均回收率为 113.72%(n=6),RSD为 1.50%,检出限为 0.027 µg/mL;叶黄素在 0.216～21.6 µg/mL范围内线性关系良好,判定系数r2=1.000 0,平均回收率为 105.96%(n=6),RSD为 3.78%,检出限为 0.056 µg/mL.该方法快速、简单稳定、结果准确,适用于同时测定枸杞提取物中叶黄素和玉米黄质.

Abstract

The chromatographic column Innoval ODS-2(4.6 mm×250 mm,5 µm)is selected with 0.05%triethylamine-acetonitrile as the mobile phase at a flow rate of 0.8 mL/min and a column temperature of 28℃.The samples are determined at 450 nm.The linear relationship of zeaxanthin is good within the range of 0.207 to 20.7 µg/mL,with a determination coefficient R2=1.000 0,an average recovery rate of 113.72%(n=6),a RSD of 1.50%,and a detection limit of 0.027 µg/mL.The linear relationship of lutein is good within the range of 0.216 to 21.6 µg/mL,with a determination coefficient R2=1.000 0,an average recovery rate of 105.96%(n=6),a RSD of 3.78%,and a detection limit of 0.056 µg/mL.The method is fast,simple,stable and accurate.It can be used for simultaneous determination of lutein and zeaxanthin in Lycium Barbarum extract.

关键词

高效液相色谱法/叶黄素/玉米黄质/枸杞提取物/基线分离

Key words

high performance liquid chromatography/lutein/zeaxanthin/Lycium barbarum extract/baseline separation

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出版年

2024

天津科技

天津科学技术信息研究所

天津科技

影响因子：0.253

ISSN：1006-8945

参考文献量23

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