Transcriptome Sequencing and Differential Expression Gene Analysis of Mantle and Gills of Crassostrea Hongkongensis Infected with Polydora
In order to explore the immune response mechanism of Crassostrea hongkongensis to the infection of polydora,the transcriptome of mantle and gill of Crassostrea hongkongensis before and after the infection of polydora were compared and analyzed by Illumina technology.The differentially expressed genes of oysters infected with polydora were annotated,and the genes related to immune metabolic response were verified.A total of 74.85 GB of clean data was obtained,including 6 923 differentially expressed genes in the infected mantle,3 770 up-regulated genes and 3 153 down regulated genes;There were 7 410 differential genes in the infected gills,3 646 up-regulated genes and 3 764 down regulated genes;4 398 and 4 780 genes were annotated on the GO classification in the mantle and gill groups of infected and healthy oysters,respectively;There were 3 851 differentially expressed genes in mantle and 4 367 differentially expressed genes in gill transcriptome in KEGG database,and most of the enriched pathways were related to the body's immunity.Five differentially expressed genes including heat shock protein 70(HSP70),42 homologues(CDC42),cytochrome P450(CYP450)and other genes related to immune metabolism were selected for fluorescence quantitative verification.The results show that the expression trend of the selected differentially expressed genes is basically consistent with the results of high-throughput sequencing.Studies show that the infection of polydora can be prevented by strengthening the synergistic effect of multiple immune metabolism genes in vivo.
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