角膜基质是角膜的主要组成部分,占整个角膜的90%,是决定角膜组织/功能的主要因素,在调节眼睛的屈光性和保护眼睛的内部结构方面起着关键作用.角膜基质细胞是角膜基质层的主要细胞类型.作为一个典型的生物力学系统,对单个细胞的力学特性的探索具有重要的生理学和病理学意义,因为细胞的力学特性是影响细胞功能和疾病发展的主要因素.细胞的生物功能高度依赖于它与基底的相互作用.因此,文中旨在利用原子力显微镜(atomic force microscopy,AFM),以纳米级的分辨率研究在不同基底上培养的角膜基质细胞的形态和力学属性.通过AFM纳米压痕技术,在细胞表面的特定位置将加载曲线与球形赫兹接触模型进行拟合,获得局部弹性模量;将卸载曲线与细胞粘附能量相联系,粘附能量是由阴影面积定义,它是AFM尖端从细胞表面分离所需的总功.结果显示,不同基底对角膜基质细胞的形态和力学特性均有所区别.文中的研究将阐明角膜生物工程以及体内组织再生的新应用.
Effects of substrate on the biomechanical properties of corneal stromal cells
The corneal stroma is the main component of the cornea,accounting for 90%of the entire cornea,and is a major determinant of corneal tissue/function,playing a key role in regulating the refractive properties of the eye and protecting the internal structure of the eye.Corneal stromal cells are the main cell type of the corneal stroma.As a typical biomechanical system,the exploration of the mechanical properties of individual cells is of great physiological and pathological importance,as the mechanical properties of the cell are a major factor influencing cell function and disease progression.The biological function of a cell is highly dependent on its interaction with the substrate.The aim of this paper is therefore to investigate the morphological and mechanical properties of corneal stromal cells cultured on different substrates using atomic force microscopy(AFM)in nanoscale resolution.The local elastic modulus is obtained by fitting the loading curve to a spherical Hertzian contact model at a specific location on the cell surface by the AFM nanoindentation technique;the unloading curve is related to the cell adhesion energy,which is defined by the shaded area and is the total work required to separate the AFM tip from the cell surface.Our results show that the different substrates differ in their morphological and mechanical properties for corneal stromal cells.Our study will shed light on new applications for corneal bioengineering as well as in vivo tissue regeneration.
corneal stromal cellsatomic force microscopynanoindentationcellular elastic modulus
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