首页|Distinct Side Population Cell Subtypes Have Different Stemness Levels in Human Ovarian Cancer Cells

Distinct Side Population Cell Subtypes Have Different Stemness Levels in Human Ovarian Cancer Cells

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The stemness of different side population(SP)cell subtypes in ovarian cancer cells was studied,and the heterogeneity of ovarian cancer stem cells was analyzed.The cisplatin-resistant human serous ovarian cancer cell line C13 was stained with the bisbenzimide Hoechst 33342.A flow cytometry-based fluorescence-activated sorting method was used to obtain lower-SP(LSP)cells,upper-SP(USP)cells,and non-SP cells(NSP)based on their sensitivity to the staining time and Hoechst dye concentration.The sphere-forming capability,expression levels of stem cell markers,resistance to high concentrations of cisplatin,and subcutaneous tumorigenicity in NOD/SCID mice of the different cell subtypes were evaluated.The C13 cells contained SP cells with stemness characteristics,and the LSP cell subtype expressed higher levels of stem cell markers,had higher in vitro sphere-forming capability,higher cisplatin resistance and higher in vivo subcutaneous tumorigenesis than USP cells(P＜0.05).NSP cells had no stemness.In conclusion,different subtypes of ovarian cancer SP cells have different stemness levels,and ovarian cancer stem cells may be heterogeneous.

ovarian cancercancer stem cellsheterogeneityside population

Yan-jie WENG、Xiao-xiao ZHANG、Xue WU、Li-li GUO、Chang-yu WANG

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Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

This project was supported by the Fundamental Research Funds for the Central Universitiesa grant from the National Natural Science Foundation of China

HUST:2017KFYXJJ12281672580

2021

10.1007/s11596-021-2327-0

当代医学科学(英文)
同济医科大学

当代医学科学(英文)

SCI
ISSN：2096-5230
年,卷(期)：2021.41(1)
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