首页|Imbalance of Circulating Follicular Regulatory and Follicular Helper T Cell Subpopulations Is Associated with Disease Progression and Serum CYFRA 21-1 Levels in Patients with Non-small Cell Lung Cancer

Imbalance of Circulating Follicular Regulatory and Follicular Helper T Cell Subpopulations Is Associated with Disease Progression and Serum CYFRA 21-1 Levels in Patients with Non-small Cell Lung Cancer

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Objective:This study aimed to investigate the changes of follicular helper T(TFH)and follicular regulatory T(TFR)cell subpopulations in patients with non-small cell lung cancer(NSCLC)and their significance.Methods:Peripheral blood was collected from 58 NSCLC patients at different stages and 38 healthy controls.Flow cytometry was used to detect TFH cell subpopulation based on programmed death 1(PD-1)and inducible co-stimulator(ICOS),and TFR cell subpopulation based on cluster determinant 45RA(CD45RA)and forkhead box protein P3(FoxP3).The levels of interleukin-10(IL-10),interleukin-17a(IL-17a),interleukin-21(IL-21),and transforming growth factor-β(TGF-β)in the plasma were measured,and changes in circulating B cell subsets and plasma IgG levels were also analyzed.The correlation between serum cytokeratin fragment antigen 21-1(CYFRA 21-1)levels and TFH,TFR,or B cell subpopulations was further explored.Results:The TFR/TFH ratio increased significantly in NSCLC patients.The CD45RA+FoxP3int TFR subsets were increased,with their proportions increasing in stages Ⅱ toⅢ and decreasing in stage Ⅳ.PD-1+ICOS+TFH cells showed a downward trend with increasing stages.Plasma IL-21 and TGF-β concentrations were increased in NSCLC patients compared with healthy controls.Plasmablasts,plasma IgG levels,and CD45RA+FoxP3int TFR cells showed similar trends.TFH numbers and plasmablasts were positively correlated with CYFRA 21-1 in stages Ⅰ-Ⅲand negatively correlated with CYFRA 21-1 in stage Ⅳ.Conclusion:Circulating TFH and TFR cell subpopulations and plasmablasts dynamically change in different stages of NSCLC,which is associated with serum CYFRA 21-1 levels and reflects disease progression.

non-small cell lung cancerfollicular helper T cellsfollicular regulatory T cellsprogression

Tian-ci LIU、Mo-han ZHENG、Xing-yue ZENG、Rui KANG、Ayibaota Bahabayi、Bulidierxin Tuerhanbayi、Song-song LU、Chen LIU

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Department of Clinical Laboratory,Peking University People's Hospital,Beijing 100044,China

School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaPeking University People's Hospital Scientific Research Development Funds

8227175581871230RZ 2022-06

2024

10.1007/s11596-023-2810-x

当代医学科学(英文)
华中科技大学同济医学院

当代医学科学(英文)

影响因子：0.748
ISSN：2096-5230
年,卷(期)：2024.44(1)
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