首页|Inhibiting MMP13 Attenuates Deep Vein Thrombosis in a Mouse Model by Reducing the Expression of Pdpn

Inhibiting MMP13 Attenuates Deep Vein Thrombosis in a Mouse Model by Reducing the Expression of Pdpn

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Objective:Matrix metalloproteinase 13(MMP13)is an extracellular matrix protease that affects the progression of atherosclerotic plaques and arterial thrombi by degrading collagens,modifying protein structures and regulating inflammatory responses,but its role in deep vein thrombosis(DVT)has not been determined.The purpose of this study was to investigate the potential effects of MMP13 and MMP13-related genes on the formation of DVT.Methods:We altered the expression level of MMP13 in vivo and conducted a transcriptome study to examine the expression and relationship between MMP13 and MMP13-related genes in a mouse model of DVT.After screening genes possibly related to MMP13 in DVT mice,the expression levels of candidate genes in human umbilical vein endothelial cells(HUVECs)and the venous wall were evaluated.The effect of MMP13 on platelet aggregation in HUVECs was investigated in vitro.Results:Among the differentially expressed genes,interleukin 1 beta,podoplanin(Pdpn),and factor Ⅷvon Willebrand factor(F8VWF)were selected for analysis in mice.When MMP13 was inhibited,the expression level of PDPN decreased significantly in vitro.In HUVECs,overexpression of MMP13 led to an increase in the expression level of PDPN and induced platelet aggregation,while transfection of PDPN-siRNA weakened the ability of MMP13 to increase platelet aggregation.Conclusions:Inhibiting the expression of MMP13 could reduce the burden of DVT in mice.The mechanism involves downregulating the expression of Pdpn through MMP13,which could provide a novel gene target for DVT diagnosis and treatment.

deep vein thrombosismatrix metalloproteinase 13podoplanin

Ji LUO、Jin ZHOU、Jing-zeng LUO、Hai-long WANG、Xue-ling ZHAO、Ru-dan ZHOU

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Department of Intensive Care Unit,The First People's Hospital of Ziyang,Ziyang 641300,China

Department of Orthopedics,Kunming Medical University Second Affiliated Hospital,Kunming 650033,China

Department of Orthopedics,Kunming Medical University First Affiliated Hospital,Kunming 650032,China

Department of Orthopedics,The Third People's Hospital of Yunnan Province,Kunming 650200,China

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General Project of Yunnan Basic Research ProgramJoint Project of Kunming Medical University and Science and Technology Department of Yunnan ProvinceJoint Project of Kunming Medical University and Science and Technology Department of Yunnan ProvinceYunnan Provincial Orthopedic and Sports Rehabilitation Clinical Medicine Research Center

202301AT070104202001AY070001-185202101AY070001-119202102AA310068

2024

10.1007/s11596-024-2862-6

当代医学科学(英文)
华中科技大学同济医学院

当代医学科学(英文)

影响因子：0.748
ISSN：2096-5230
年,卷(期)：2024.44(2)
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